caa a22 4500 465734324 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01189075 doi (NATIONALLICENCE)springer-10.1007/BF01189075 Structures of acidic O-linked polylactosaminoglycans on human skim milk mucins [Elektronische Daten] [Franz-Georg Hanisch, Jasna Peter-Katalinic, Heinz Egge, Ursula Dabrowski, Gerhard Uhlenbruck] O-Linked glycans were isolated from human skim milk mucins or mucin-derived high-molecular weight glycopeptides and fractionated by anion exchange chromatography into neutral and acidic alditols. Major oligosaccharides contained in the acidic fraction were purified by high performance liquid chromatography and structurally characterized by a combination of fast atom bombardment mass spectrometry, methylation analysis and 500 MHz1H-nuclear magnetic resonance spectroscopy. The structural aspects exhibited by these major species in the acidic fraction resemble those established previously for the neutral oligosaccharides from human skim milk mucins: 1) the size of the alditols varies from tri- to decasaccharides 2) the core structure is of the ubiquitous type 2 3) the backbone sequences are of the poly-N-acetyllactosamine type with a particular preponderance of linearly extended GlcNAcβ(1-3)Gal (major) or GlcNAcβ(1-6)Gal units (minor). N-Acetylneuraminic acid on monosialylated (mucin- or glycopeptide-derived) and disialylated glycans (glycopeptide derived) is linked predominantly to position C-3 of galactose. Glycoconjugate Journal, 1990 O-linked glycans nationallicence acidic carbohydrates nationallicence mucins nationallicence human milk nationallicence FAB mass spectrometry nationallicence NMR spectroscopy nationallicence Hanisch Franz-Georg Institut für Immunbiologie, Universitätskliniken Köln, Kerpener Strasse 15, D-Köln 41, Germany aut Peter-Katalinic Jasna Institut für Physiologische Chemie, Universität Bonn, Nussallee 11, D-5300, Bonn 1, Germany aut Egge Heinz Institut für Physiologische Chemie, Universität Bonn, Nussallee 11, D-5300, Bonn 1, Germany aut Dabrowski Ursula Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, D-6900, Heidelberg, Germany aut Uhlenbruck Gerhard Institut für Immunbiologie, Universitätskliniken Köln, Kerpener Strasse 15, D-Köln 41, Germany aut Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 525-543 0282-0080 7:6<525 1990 7 10719 https://doi.org/10.1007/BF01189075 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01189075 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hanisch Franz-Georg Institut für Immunbiologie, Universitätskliniken Köln, Kerpener Strasse 15, D-Köln 41, Germany aut NATIONALLICENCE 700 1- Peter-Katalinic Jasna Institut für Physiologische Chemie, Universität Bonn, Nussallee 11, D-5300, Bonn 1, Germany aut NATIONALLICENCE 700 1- Egge Heinz Institut für Physiologische Chemie, Universität Bonn, Nussallee 11, D-5300, Bonn 1, Germany aut NATIONALLICENCE 700 1- Dabrowski Ursula Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, D-6900, Heidelberg, Germany aut NATIONALLICENCE 700 1- Uhlenbruck Gerhard Institut für Immunbiologie, Universitätskliniken Köln, Kerpener Strasse 15, D-Köln 41, Germany aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 525-543 0282-0080 7:6<525 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer