caa a22 4500 465734332 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01189081 doi (NATIONALLICENCE)springer-10.1007/BF01189081 Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein) [Elektronische Daten] [Rosalita Smagula, Herman Van Halbeek, Jean Decker, Andrew Muchmore, Charles Moody, Anne Sherblom] The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hessionet al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]. We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2-2 μM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmoreet al., J Leukocyte Biol (in press)]. In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources. Surprisingly, there was a pregnancy-associated decrease in the total content of oligomannose chains due predominantly to a reduction in Man5GlcNAc2-R and Man6GlcNAc2-R. Man7GlcNAc2-R, which did not decrease with pregnancy, comprised a significantly greater proportion of the total oligomannose chains in pregnant vs. nonpregnant samples from both species (human; 34.6% vs. 25.9%: bovine; 14.4% vs. 7.2%). Glycoconjugate Journal, 1990 uromodulin nationallicence Tamm-Horsfall glycoprotein nationallicence pregnancy nationallicence immunosuppression nationallicence oligomannose oligosaccharides nationallicence Smagula Rosalita Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut Van Halbeek Herman Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602, Athens, GA, USA aut Decker Jean Metabolism Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 6B09, 20892, Bethesda, MD, USA aut Muchmore Andrew Metabolism Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 6B09, 20892, Bethesda, MD, USA aut Moody Charles Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut Sherblom Anne Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 609-624 0282-0080 7:6<609 1990 7 10719 https://doi.org/10.1007/BF01189081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01189081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smagula Rosalita Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut NATIONALLICENCE 700 1- Van Halbeek Herman Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Decker Jean Metabolism Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 6B09, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Muchmore Andrew Metabolism Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 6B09, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Moody Charles Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut NATIONALLICENCE 700 1- Sherblom Anne Department of Biochemistry and Microbiology, University of Maine, Hitchner Hall, 04469, Orono, ME, USA aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 609-624 0282-0080 7:6<609 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer