caa a22 4500 465734359 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01189078 doi (NATIONALLICENCE)springer-10.1007/BF01189078 Purification of the blood group H gene associated α-2- l -fucosyltransferase from human plasma [Elektronische Daten] [Philip Kyprianou, Alan Betteridge, Alastair Donald, Winifred Watkins] The α-2-l-fucosyltransferase in human plasma has been freed from α-3-l-fucosyltransferase activity and purified approximately 200,000-fold by a series of steps involving ammonium sulphate precipitation, hydrophobic chromatography on Phenyl Sepharose 4B and affinity chromatography first on GDP-adipate-Sepharose and then on GDP-hexanolamine-Sepharose. The purified α-2-l-fucosyltransferase had a Mr on gel filtration HPLC of 158,000 and showed optimal activity in the pH range 6.5-7.0. The enzyme transferred fucose equally well to Type 1 (Galβ1-3GlcNAc) and Type 2 (Galβ1-4GlcNAc) substrates but Type 3 (Galβ1-3GalNAc) structures were less efficient acceptors. Competition experiments indicated that a single enzyme species in the purified preparation was responsible for reactivity with the Type 1 and Type 2 structures. Thus the differences in conformation between the Type 1 and Type 2 disaccharides do not appear to influence the capacities of their terminal non-reducing β-d-galactosyl residues to function as acceptor substrates for the α-2-l-fucosyltransferase expressed by the blood groupH gene in haemopoietic tissue. Glycoconjugate Journal, 1990 α-2-fucosyltransferase nationallicence H gene nationallicence blood group H nationallicence Se gene nationallicence human plasma nationallicence Kyprianou Philip Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, NW1 OTU, London, UK aut Betteridge Alan Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, NW1 OTU, London, UK aut Donald Alastair Division of Immunochemical Genetics, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut Watkins Winifred Division of Immunochemical Genetics, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 573-588 0282-0080 7:6<573 1990 7 10719 https://doi.org/10.1007/BF01189078 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01189078 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kyprianou Philip Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, NW1 OTU, London, UK aut NATIONALLICENCE 700 1- Betteridge Alan Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, NW1 OTU, London, UK aut NATIONALLICENCE 700 1- Donald Alastair Division of Immunochemical Genetics, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut NATIONALLICENCE 700 1- Watkins Winifred Division of Immunochemical Genetics, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 573-588 0282-0080 7:6<573 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer