caa a22 4500 465734383 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01050609 doi (NATIONALLICENCE)springer-10.1007/BF01050609 The role of carbohydrate in the blood group N-related epitopes recognised by three new monoclonal antibodies [Elektronische Daten] [Ewa Jaskiewicz, Elwira Lisowska, Arne Lundblad] The specificity of three new monoclonal anti-glycophorin antibodies, reacting preferentially with blood group N antigen, was characterized by means of untreated, enzymatically and chemically modified M and N glycoproteins. All antibodies recognized the NH2-terminal Leu residue and its amino group, but differed in some other features, including the role of carbohydrate in the epitopes. One of the antibodies (631/3B4, IgM) showed an unusual two-directional dependence of activity on the degree of antigen desialylation. The progressive desialylation of N glycoprotein first caused a strongly increased binding to the epitope, followed by a complete loss of activity. The epitopes for the two remaining antibodies (648/4B5 and 650/4B5, both IgG1) showed reactivity independent of sialylation, but dependent on the presence of Gal-GalNAc-units. Release of the disaccharide byO-glycanase treatment of N glycoprotein abolished its reactivity with both antibodies. Glycoconjugate Journal AB, 1990 glycophorin nationallicence blood group N nationallicence monoclonal anti-N-antibodies nationallicence O-linked glycan nationallicence sialic acid's role in epitope nationallicence Jaskiewicz Ewa Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland aut Lisowska Elwira Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland aut Lundblad Arne Department of Clinical Chemistry, University Hospital, 221 85, Lund, Sweden aut Glycoconjugate Journal Kluwer Academic Publishers 7/3(1990-05-01), 255-268 0282-0080 7:3<255 1990 7 10719 https://doi.org/10.1007/BF01050609 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01050609 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jaskiewicz Ewa Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Lisowska Elwira Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Lundblad Arne Department of Clinical Chemistry, University Hospital, 221 85, Lund, Sweden aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/3(1990-05-01), 255-268 0282-0080 7:3<255 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer