caa a22 4500 465734448 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01073373 doi (NATIONALLICENCE)springer-10.1007/BF01073373 Structure and properties of a Cephalosporium acremonium α-galactosidase [Elektronische Daten] [Olga Zaprometova, Irene Ulezlo, Vladimir Lakhtin] The amino acid and sugar composition of the enzyme protein, the effect of urea, sodium dodecyl sulphate and Concanavalin A on the purified α-galactosidase (EC 3.2.1.22) from the moldCephalosporium acremonium has been studied. The results obtained by gas liquid chromatography indicated the presence ofN-acetylglucosamine, mannose, galactose andN-acetylneuramic acid in the molar proportions 2∶7∶3∶11. The presence of two types of Asn-linked oligosaccharide structures in the enzyme molecule is assumed. The α-galactosidase liberates α(1-3), α(1-4) and α(1-6)-linkedd-galactose units from various synthetic and natural substrates which have been tested. The effects of pH, substrate concentration and temperature on the catalytic activity of the enzyme are described. The purified α-galactosidase also exhibited a lectin activity with an affinity towards glucose, and to some extent mannose. Glycoconjugate Journal AB, 1990 α-galactosidase (EC 3.2.1.22) nationallicence Cephalosporium acremonium nationallicence lectin (hemagglutinin) activity nationallicence glycoprotein structure nationallicence p-NPG : p-nitrophenyl-α-d-galactopyranoside nationallicence 4-MUG : 4-methylumbelliferyl-α-d-galactopyranoside nationallicence HU : hemagglutinin unit nationallicence PBS : phosphate buffered saline nationallicence SDS : sodium dodecyl sulphate nationallicence ConA : Concanavalin A nationallicence WGA : wheat germ agglutinin nationallicence LCA : Lens culinaris agglutinin nationallicence PHA : phytohemagglutinin fromPhaseolus vulgaris nationallicence Zaprometova Olga A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R. aut Ulezlo Irene A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R. aut Lakhtin Vladimir A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R. aut Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 287-300 0282-0080 7:4<287 1990 7 10719 https://doi.org/10.1007/BF01073373 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01073373 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zaprometova Olga A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R aut NATIONALLICENCE 700 1- Ulezlo Irene A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R aut NATIONALLICENCE 700 1- Lakhtin Vladimir A N Bakh Institute of Biochemistry, Academy of Sciences of the USSR, Leninsky Prospekt 33, 117071, Moscow, U.S.S.R aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 287-300 0282-0080 7:4<287 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer