caa a22 4500 465734456 CHVBK 20180323111751.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01073375 doi (NATIONALLICENCE)springer-10.1007/BF01073375 Structural requirements for the binding of oligosaccharides to immobilized lectin of Erythrina variegata (Linn) var. Orientalis [Elektronische Daten] [Hua Li, Kazuo Yamamoto, Hiroto Kawashima, Toshiaki Osawa] The structural requirements for the interaction of asparagine-linked oligosaccharide moieties of glycoproteins withErythrina variegata agglutinin (EVA) were investigated by means of affinity chromatography on an EVA-Sepharose column. Some of the branched poly-N-acetyllactosamine-type oligosaccharides obtained from human erythrocyte band 3 glycoprotein were found to show high affinity to EVA-Sepharose, whereas complex-type oligosaccharides were shown to have low affinity. Hybrid type, oligomannose-type and unbranched poly-N-acetyllactosamine-type oligosaccharides bound very little or not at all to EVA-Sepharose. To further study the carbohydrate-binding specificity of this lectin, we investigated the interaction of immobilized EVA and oligosaccharide fragments obtained through partial hydrolysis from branched poly-N-acetyllactosamine-type oligosaccharides. Branched poly-N-acetyllactosamine-type oligosaccharides were subjected to limited hydrolysis with 0.1% trifluoroacetic acid at 100°C for 40 min and then separated on an amino-bonded silica column. One of pentasaccharides thus prepared strongly bound to the EVA-Sepharose column. Structural analysis of this pentasaccharide showed that the Galβ1-4GlcNAcβ1-3(Galβ1-4GlcNAcβ1-6)Gal sugar sequence, which is an l-antigen determinant, was essential for the high affinity binding of the oligosaccharides to the EVA-Sepharose column. Glycoconjugate Journal AB, 1990 oligosaccharide binding nationallicence immobilized lectin nationallicence Erythrina nationallicence EVA : Erythrina variegata agglutinin nationallicence WGA : wheat germ agglutinin nationallicence STA : potato lectin nationallicence LEA : tomato lectin nationallicence DSA : Datura stramonium agglutinin nationallicence PBS : 0.01 M sodium phosphate buffer, pH 7.3, containing 0.15 M NaCl nationallicence Galol : galactitol nationallicence Li Hua Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Yamamoto Kazuo Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Kawashima Hiroto Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 311-322 0282-0080 7:4<311 1990 7 10719 https://doi.org/10.1007/BF01073375 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01073375 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hua Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamamoto Kazuo Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawashima Hiroto Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 311-322 0282-0080 7:4<311 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer