caa a22 4500 465734502 CHVBK 20180323111751.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01073374 doi (NATIONALLICENCE)springer-10.1007/BF01073374 Dall'Olio Fabio Dipartimento di Patologia Sperimentale dell'Università di Bologna, Via S. Giacomo 14, 40126, Bologna, Italy aut Sialyltransferases of developing rat brain [Elektronische Daten] [Fabio Dall'Olio] The activity of four different sialyltransferases acting on N- or O-linked chains of glycoproteins was studied in brains of 19 days-old embryos, 1 day-old newborns and adult rats. By using asialofetuin, fetuin andN-acetyllactosamine as acceptors, it has been possible to measure independently the following enzyme activities: CMP-NeuAc:Galβ1-3GalNac α(2-3)-sialyltransferase (EC 2.4.99.4), CMP-NeuAc:Galβ1-4GlcNAc α(2-3)-sialyltransferase (EC 2.4.99.6), CMP-NeuAc:Galβ1-4GlcNAc α(2-6)-sialyltransferase (EC 2.4.99.1) and CMP-NeuAc:NeuAcα2-3Galβ1-3GalNac α(2-6)-sialyltransferase (EC 2.4.99.7). The specific activity of the first three enzymes which act on asialylated acceptors showed a 2.6-fold decrease in a parallel manner after ontogenic development, while the activity of NeuAcα2-3Galβ1-3GalNac α(2-6)-sialyltransferase was four times lower in adult than in embryonic brain, showing a stronger dependence on ontogenic development. Despite the higher level of sialyltransferases able to act on glycoproteins, in fetal brain these glycoproteins do not contain a higher amount of sialic acid. Glycoconjugate Journal AB, 1990 brain sialyltransferases nationallicence fetuin nationallicence development nationallicence glycosyltransferases nationallicence HPLC : high performance liquid chromatography nationallicence N-CAM : neural cell adhesion molecule nationallicence Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 301-310 0282-0080 7:4<301 1990 7 10719 https://doi.org/10.1007/BF01073374 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01073374 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Dall'Olio Fabio Dipartimento di Patologia Sperimentale dell'Università di Bologna, Via S. Giacomo 14, 40126, Bologna, Italy aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 301-310 0282-0080 7:4<301 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer