caa a22 4500 465734529 CHVBK 20180323111751.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01073376 doi (NATIONALLICENCE)springer-10.1007/BF01073376 Carbohydrate binding specificities of several poly- N -acetyllactosamine-binding lectins [Elektronische Daten] [Hiroto Kawashima, Shinobu Sueyoshi, Hua Li, Kazuo Yamamoto, Toshiaki Osawa] The structural requirements for the interaction of the Asn-linked poly-N-acetyllactosamine-type oligosaccharide moieties of glycoproteins with variousN-acetylglucosamine-binding lectins were investigated by means of affinity chromatography on immobilized lectin-Sepharose columns. High molecular weight glycopeptides containing poly-N-acetyllactosamine-type oligosaccharides obtained by Pronase digestion of human erythrocyte ghosts were treated with 0.1 M trifluoroacetic acid at 100°C for 40 min and then several oligosaccharide fragments were purified with an amino-bonded silica column. Among these oligosaccharide fragments, trisaccharide Galβ1-4GlcNAcβ1-6Galol bound to the wheat germ agglutinin (WGA)- and pokeweed mitogen (PWM)-Sepharose columns, and also showed affinity to theDatura stramonium agglutinin (DSA)-,Lycopersicon esculentum (tomato) agglutinin-andSolanum tuberosum (potato) agglutinin-Sepharose columns. Pentasaccharide Galβ1-4GlcNAcβ1-3(Galβ1-4GlcNAcβ1-6)Galol showed weaker affinity to the WGA- and PWM-Sepharose columns, compared to the trisaccharide. Trisaccharide GlcNAcβ1-3(GlcNAcβ1-6)Galol showed weak affinity to the WGA-Sepharose column and did not show any affinity to the other lectin-Sepharose columns. Hexasaccharide Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcol bound only to the DSA-Sepharose column, indicating that only DSA does not require a GlcNAcβ(1-6)-linkage for interaction. Glycoconjugate Journal AB, 1990 Kawashima Hiroto Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Sueyoshi Shinobu Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Li Hua Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Yamamoto Kazuo Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 323-334 0282-0080 7:4<323 1990 7 10719 https://doi.org/10.1007/BF01073376 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01073376 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawashima Hiroto Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Sueyoshi Shinobu Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Li Hua Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamamoto Kazuo Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Osawa Toshiaki Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/4(1990-07-01), 323-334 0282-0080 7:4<323 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer