caa a22 4500 465735185 CHVBK 20180323111753.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02341150 doi (NATIONALLICENCE)springer-10.1007/BF02341150 Effects of131I-EGF on cultured human glioma cells [Elektronische Daten] [Jacek Capala, Marianne Pråhl, Steve Scott-Robson, Jan Pontén, Bengt Westermark, Jörgen Carlsson] Malignant glioma cells often have more epidermal growth factor (EGF) receptors than normal cells and targeting of toxic substances to the receptor might therefore be an attractive therapeutical approach. Radiation effects were analysed on human glioma cells growing as monolayers after exposure to131I-EGF. Unspecific effects were analysed with131I-BSA or after presaturation with nonradioactive EGF. The radiation effects were compared to the effects obtained by external60Co gamma irradiation. Administration of the highest radioactive concentrations, 0.2-0.5 MBq/ml in the culture medium, corresponded, after 20 min incubation, to a binding of about 1.0-2.5 dpm/cell. Such an exposure to131I decays gave effects on cell survival corresponding to about 2.5 Gy of external gamma irradiation. Somewhat less than half of this effect came from the specific bound radioactivity and the rest from nonbound radioactivity. When administrating lower concentrations of radioactivity both the binding and the radiation effects were smaller. The observations showed that it is possible to inactivate cell-proliferation of glioma cells with specific bound131I-EGF. The possibilities to fractionate the treatments and of binding also other toxic agents than131I to the EGF receptor are discussed. Kluwer Academic Publishers, 1990 clonogenic survival nationallicence cultured cells nationallicence EGF nationallicence EGF-receptor nationallicence epidermal growth factor nationallicence glioma nationallicence iodine nationallicence radiation effects nationallicence Capala Jacek Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut Pråhl Marianne Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut Scott-Robson Steve Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut Pontén Jan Department of Pathology, Uppsala University and the Academical hospital, 75185, Uppsala, Sweden aut Westermark Bengt Department of Pathology, Uppsala University and the Academical hospital, 75185, Uppsala, Sweden aut Carlsson Jörgen Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 201-210 0167-594X 9:3<201 1990 9 11060 https://doi.org/10.1007/BF02341150 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02341150 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Capala Jacek Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Pråhl Marianne Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Scott-Robson Steve Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Pontén Jan Department of Pathology, Uppsala University and the Academical hospital, 75185, Uppsala, Sweden aut NATIONALLICENCE 700 1- Westermark Bengt Department of Pathology, Uppsala University and the Academical hospital, 75185, Uppsala, Sweden aut NATIONALLICENCE 700 1- Carlsson Jörgen Department of Radiation Sciences, Uppsala University, Box 535, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 201-210 0167-594X 9:3<201 1990 9 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer