caa a22 4500 465735231 CHVBK 20180323111753.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02341157 doi (NATIONALLICENCE)springer-10.1007/BF02341157 Factor analysis of the immunophenotypes of astrocytomas and malignant gliomas: Correlations with tumor grade and patient survival [Elektronische Daten] [Mark Jennings, Laura Asadourian, V. Jennings, Joan Shapiro, H. Thaler] Summary: Our previous investigations correlated the degree of cytogenetic and immunophenotypic heterogeneity of cultured normal glia, astrocytomas and malignant gliomas. The possible significance was suggested by the statistical correlation of individual antigens with diagnosis and patient survival [1]. The present study has established the patterns of covariation of titers of monoclonal antibody reactivity with a panel of cell surface antigens among normal glia (8), astrocytomas (4), anaplastic astrocytomas (12), mixed malignant gliomas (8) and glioblastomas (21). A mean aggregate titer across 43 antigens was computed for each culture and then subtracted from the observed individual titers. Factor analysis was performed to determine a small number of Factors, derived as the weighted average of the 43 mean-adjusted antigens, which accounted for a significant proportion of the covariation of immunophenotypic expression in the sample of 53 cultures. Clusters of antigens were found to independently segregate in their deviation from the aggregate phenotype. Adjusting for age and diagnosis, Factors 1 and 4 correlated with patient survival among recurrent and primary neoplasms, respectively. Factor 2 additionally discriminated between primary and recurrent gliomas. Factor 3 was associated with age at diagnosis. Factors 1 and 2 correlated with the histopathologic grade of glial tumor. Scatter plots of Factor 1 vs. 2 revealed the minimal immunophenotypic diversity of the normal glia. Astrocytomas were similar but not identical. Progressive divergence was evident between the immunophenotypes of anaplastic astrocytomas, mixed gliomas and glioblastomas. These data suggest that qualitative and quantitative differences in antigenic heterogeneity may identify stages in glial tumor progression. Furthermore, the aggregate immunophenotype may be a useful prognostic indicator, which is independent of established clinical variables such as age at diagnosis and pathologic grade of neoplasm. Factor analysis has been shown to be an efficient means of reducing the dimensionality of the data without sacrificing its informative character. Kluwer Academic Publishers, 1990 immunophenotypes nationallicence astrocytomas nationallicence malignant gliomas nationallicence factor analysis nationallicence Jennings Mark Departments of Neurology and Pediatrics, Vanderbilt University, 2100 Pierce Ave., 37212, Nashville, TN aut Asadourian Laura Human Cancer Immunology Laboratory, USA aut Jennings V. aut Shapiro Joan George Cotzias Laboratory of Neuro-Oncology, USA aut Thaler H. Department of Biostatistics, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., 10021, New York, NY, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 265-273 0167-594X 9:3<265 1990 9 11060 https://doi.org/10.1007/BF02341157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02341157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jennings Mark Departments of Neurology and Pediatrics, Vanderbilt University, 2100 Pierce Ave., 37212, Nashville, TN aut NATIONALLICENCE 700 1- Asadourian Laura Human Cancer Immunology Laboratory, USA aut NATIONALLICENCE 700 1- Jennings V. aut NATIONALLICENCE 700 1- Shapiro Joan George Cotzias Laboratory of Neuro-Oncology, USA aut NATIONALLICENCE 700 1- Thaler H. Department of Biostatistics, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., 10021, New York, NY, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 265-273 0167-594X 9:3<265 1990 9 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer