caa a22 4500 46573524X CHVBK 20180323111753.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02341149 doi (NATIONALLICENCE)springer-10.1007/BF02341149 Selective cytoplasmic and membrane changes induced by cisplatinum [Elektronische Daten] [Mary Oberc-Greenwood, Barry Smith, Carol Cooke, Cathy Pepin, Paul Kornblith] Summary: Cisplatinum (cis-dichlorodiammineplatinum II(NSC-119875)), proven to be of therapeutic value in a variety of solid tumors, is thought to have DNA as its major target. Priorin vitro studies have suggested that it also induces cell membrane and cytoplasmic changes. To better understand glial tumor cell sensitivity to cisplatinum and to design more effective adjuvant therapy, three cisplatinum sensitive human glioma-derived cell lines, SNB-1, SNB-3, SNB-4, were examined by transmission electron microscopy for cisplatinum induced changes. Tumor cells were exposed to 25, 50, and 100 µg/ml cisplatinum in medium for varying time periods (4-72 hours). Four changes were consistent: cell rounding and reduced nuclear-cytoplasmic ratio, nuclear chromatin clumping, vesiculation and swelling of the golgi apparatus, and dilatation of the smooth endoplasmic reticulum. These morphologic changes are distinct for cisplatinum and unlike those induced by BCNU (plasma membrane blebbing) and AZQ (mitochondrial swelling and destruction) previously seen in our laboratory. The cellular events described here suggest that cytoplasmic, as well as nuclear, changes (occurring within the same time intervals) may both be relevant to the antitumor effects of cisplatinum. Kluwer Academic Publishers, 1990 cisplatinum nationallicence golgi apparatus nationallicence endoplasmic reticulum nationallicence cytotoxicity nationallicence Oberc-Greenwood Mary Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut Smith Barry Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut Cooke Carol Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut Pepin Cathy Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut Kornblith Paul Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 191-199 0167-594X 9:3<191 1990 9 11060 https://doi.org/10.1007/BF02341149 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02341149 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oberc-Greenwood Mary Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut NATIONALLICENCE 700 1- Smith Barry Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut NATIONALLICENCE 700 1- Cooke Carol Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut NATIONALLICENCE 700 1- Pepin Cathy Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut NATIONALLICENCE 700 1- Kornblith Paul Surgical Neurology Branch, NINCDS National Institutes of Health Bethesda, 20892, MD, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 9/3(1990-12-01), 191-199 0167-594X 9:3<191 1990 9 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer