caa a22 4500 465735495 CHVBK 20180323111753.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00182081 doi (NATIONALLICENCE)springer-10.1007/BF00182081 Platelet Derived Growth Factor (PDGF) autocrine components in human tumor cell lines [Elektronische Daten] [Griffith Harsh, Mark Keating, Jaime Escobedo, Lewis Williams] Tumor cells may stimulate their own proliferation through an autocrine mechanism by simultaneously producing growth factors and growth factor receptors. We now report that numerous human tumor-derived cell lines simultaneously express the genes for platelet-derived growth factor (PDGF) A and B chains and the PDGF receptor (PDGF-R). Measurement of mRNA transcribed from these genes showed that among 16 malignant glioma cell lines tested, 15 expressed the PDGF A gene, 12 expressed the PDGF B gene, and 13 expressed the PDGF-R gene. Of three osteosarcoma lines, three expressed PDGF A, two expressed PDGF B, and three expressed PDGF-R. For eight malignant melanoma lines, seven expressed PDGF A, five expressed PDGF B, and three expressed PDGF-R genes. Thus, 13 of 16 malignant glioma, 3 of 3 osteosarcomas, and 3 of 8 malignant melanoma cell lines expressed the PDGF receptor gene and either or both PDGF genes. Five cell lines were tested for production of biologically active PDGF and PDGF receptor protein. Media conditioned by each of the five cell lines induced tyrosine phosphorylation of a protein identical in size to the PDGF receptor. These five cell lines also produced PDGF receptor protein as measured by Western blot analysis or metabolic labeling and immunoprecipitation using PDGF-R antibodies. The PDGF receptors of these cell lines were activated by human platelet PDGF or by recombinant AA or BB homodimers. Intracellular interaction of these receptors with the growth factor simultaneously produced may provide continuous stimulation to the proliferation of these cells. Kluwer Academic Publishers, 1990 platelet derived growth factor nationallicence malignant glioma nationallicence autocrine components nationallicence oncogenes nationallicence messenger RNA nationallicence Harsh Griffith The Brain Tumor Research Center of the Department of Neurological Surgery, University of California, 94143, San Francisco, California aut Keating Mark Cardiovascular Research Institute, University of California, 94143, San Francisco, California aut Escobedo Jaime Howard Hughes Medical Institute, School of Medicine, University of California, 94143, San Francisco, California aut Williams Lewis Cardiovascular Research Institute, University of California, 94143, San Francisco, California aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/1(1990-02-01), 1-12 0167-594X 8:1<1 1990 8 11060 https://doi.org/10.1007/BF00182081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00182081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harsh Griffith The Brain Tumor Research Center of the Department of Neurological Surgery, University of California, 94143, San Francisco, California aut NATIONALLICENCE 700 1- Keating Mark Cardiovascular Research Institute, University of California, 94143, San Francisco, California aut NATIONALLICENCE 700 1- Escobedo Jaime Howard Hughes Medical Institute, School of Medicine, University of California, 94143, San Francisco, California aut NATIONALLICENCE 700 1- Williams Lewis Cardiovascular Research Institute, University of California, 94143, San Francisco, California aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/1(1990-02-01), 1-12 0167-594X 8:1<1 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer