caa a22 4500 46573555X CHVBK 20180323111754.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00182082 doi (NATIONALLICENCE)springer-10.1007/BF00182082 Spontaneous phenotypic and karyotypic progression in the SV40 transfected cell line SVG during prolonged passage in vitro [Elektronische Daten] [P. Davey, A. Rauth, L. Mason, L. Addy] Summary: Transfection of primary cultures of human cells with origin of replication deficient SV40 DNA has been carried out by others to generate in vitro models of malignant transformation in vivo. The present work describes progressive alterations in karyotype and phenotype in one such transfected (neuroglial) cell line (SVG). After repeated passage, recognisable marker chromosomes evolved. These may be related to karyotypic anomalies found in human glial tumors. Accompanying the evolution in karyotype were changes in phenotype. Although presaging malignant transformation, these stopped short of actual tumorigenicity. Kluwer Academic Publishers, 1990 astrocytes nationallicence SV40 nationallicence malignant transformation nationallicence Davey P. The Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut Rauth A. The Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut Mason L. Department of Pathology, The Princess Margaret Hospital, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut Addy L. Department of Cytogenetics, The Wellesley Hospital, 160 Wellesley Street East, M4Y IJ3, Toronto, Ontario, Canada aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/1(1990-02-01), 13-22 0167-594X 8:1<13 1990 8 11060 https://doi.org/10.1007/BF00182082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00182082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davey P. The Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Rauth A. The Ontario Cancer Institute, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Mason L. Department of Pathology, The Princess Margaret Hospital, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Addy L. Department of Cytogenetics, The Wellesley Hospital, 160 Wellesley Street East, M4Y IJ3, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/1(1990-02-01), 13-22 0167-594X 8:1<13 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer