caa a22 4500 465735592 CHVBK 20180323111754.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00177357 doi (NATIONALLICENCE)springer-10.1007/BF00177357 Rapid estimation of the proliferating index of brain tumours [Elektronische Daten] [A. Detta, E. Hitchcock] Summary: A rapid and versatile method has been developed for estimating proliferating/labelling indices (enumerated S-phase cells) of brain tumours. Fragments of fresh, surgically excised tumour specimens are labelled in vitro with 10 µM bromodeoxyuridine (BrdU) at 37° for 1 hour in MEM + 10% FCS, washed in PBS, blotted dry and then imprinted on glass slides leaving a mirror image monolayer of cells of the impressed surface. The incorporated BrdU is then detected indirectly by immunoperoxidase staining after fixation. No fewer than 1000 cells are counted under high power and the PI is expressed as the % of BrdU positive cells to the total number counted. The PI of the various tumours analysed ranged 2.54-13.08% for gliomas, 5.29 - 7.60% for metastases, 0 - 0.83% for benign meningiomas. Thirty one cases have been studied. Results, obtainable within a few hours of tumour biopsy/resection, correlate favourably with published flowcytometry proliferating indices for the tumours analysed and thus validate the method as a comparable alternative to in vivo BrdU labelling. Kluwer Academic Publishers, 1990 bromodeoxyuridine nationallicence brain neoplasm nationallicence cell proliferation nationallicence monoclonal antibody nationallicence immunocytochemistry nationallicence Detta A. University Brain Tumour Laboratory, Department of Neurosurgery, University of Birmingham, Midland Centre for Neurosurgery and Neurology, B67 7JX, Smethwick, West Midlands, United Kingdom aut Hitchcock E. University Brain Tumour Laboratory, Department of Neurosurgery, University of Birmingham, Midland Centre for Neurosurgery and Neurology, B67 7JX, Smethwick, West Midlands, United Kingdom aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/3(1990-06-01), 245-253 0167-594X 8:3<245 1990 8 11060 https://doi.org/10.1007/BF00177357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00177357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Detta A. University Brain Tumour Laboratory, Department of Neurosurgery, University of Birmingham, Midland Centre for Neurosurgery and Neurology, B67 7JX, Smethwick, West Midlands, United Kingdom aut NATIONALLICENCE 700 1- Hitchcock E. University Brain Tumour Laboratory, Department of Neurosurgery, University of Birmingham, Midland Centre for Neurosurgery and Neurology, B67 7JX, Smethwick, West Midlands, United Kingdom aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/3(1990-06-01), 245-253 0167-594X 8:3<245 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer