caa a22 4500 465735665 CHVBK 20180323111754.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00177356 doi (NATIONALLICENCE)springer-10.1007/BF00177356 Human cerebral endothelium: Isolation and characterization of cells derived from microvessels of non-neoplastic and malignant glial tissue [Elektronische Daten] [Penny Costello, Rolando Del Maestro] Human microvessels were isolated and cultured from non-neoplastic cerebral tissue specimens resected for the treatment of seizure disorders and from malignant glial tumors. After 1-2 weeks, cobblestone patterned plaques of cells were isolated and cultured from these microvessels. Cell lines positive for Factor VIII antigen and negative for glial fibrillary acidic protein were designated as endothelium. Endothelium from both tissue sources produced γ-glutamyl transpeptidase in response to glial cell conditioned media. Tumor derived microvessel endothelium had decreased longevity in culture when compared to normal microvessel endothelium. Tumor derived endothelium also formed less extensive intercellular junctional complexes in vitro. The isolation and characterization of human cerebral microvessel endothelium derived from non-neoplastic tissue and glial tumors may lead to a further understanding of the role of endothelium in tumor growth and vascular permeability alterations. Kluwer Academic Publishers, 1990 microvessels nationallicence human cerebral endothelium nationallicence malignant glioma nationallicence Costello Penny Brain Research Laboratories, Experimental Research Unit, Department of Clinical Neurological Sciences and Biochemistry, University of Western Ontario, Victoria Hospital, London, Ontario, Canada aut Del Maestro Rolando Brain Research Laboratories, Experimental Research Unit, Department of Clinical Neurological Sciences and Biochemistry, University of Western Ontario, Victoria Hospital, London, Ontario, Canada aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/3(1990-06-01), 231-243 0167-594X 8:3<231 1990 8 11060 https://doi.org/10.1007/BF00177356 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00177356 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costello Penny Brain Research Laboratories, Experimental Research Unit, Department of Clinical Neurological Sciences and Biochemistry, University of Western Ontario, Victoria Hospital, London, Ontario, Canada aut NATIONALLICENCE 700 1- Del Maestro Rolando Brain Research Laboratories, Experimental Research Unit, Department of Clinical Neurological Sciences and Biochemistry, University of Western Ontario, Victoria Hospital, London, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/3(1990-06-01), 231-243 0167-594X 8:3<231 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer