caa a22 4500 465735746 CHVBK 20180323111754.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00177834 doi (NATIONALLICENCE)springer-10.1007/BF00177834 Human neuroblastoma cell growth in xenogeneic hosts: comparison of T cell-deficient and NK-deficient hosts, and subcutaneous or intravenous injection routes [Elektronische Daten] [William Turner, Jane Chatten, Lois Lampson] We have examined two features of neuroblastoma cells that had not been well-characterized in a xenogeneic model: The cells display unusual immunologic properties in other experimental systems, and the original tumors display widespread and characteristic patterns of metastasis. To determine the most appropriate immunodeficient host for primary tumor growth, T cell-deficient nude mice, NK-deficient beige mice, beige-nudes, and controls were injected with the well-characterized line CHP-100. To define the pattern of tumor spread, complete autopsies were performed following subcutaneous, intraperitoneal and intravenous injections. CHP-100 consistently formed subcutaneous tumors in T cell-deficient mice (nude and beige-nude), but not in T cell-competent mice (beige, heterozygous nu/+ and bg/+, or wild-type). The growth rate and final size of the subcutaneous tumors were not greater in beige-nudes than in nudes. All mice showed early CHP-100 cell death after subcutaneous injection; the nature of the immunodeficiency was more relevant for the surviving subpopulation. Widespread dissemination was seen following intravenous injection, particularly in beige-nudes. Aspects of the growth patterns were appropriate to the tumor of origin. The behavior in immunodeficient mice suggests that T cells can play a role in controlling the growth of these cells; the next steps will be to define the effector mechanisms, and to determine if they can be exploited for human patients. The hematogenous spread following intravenous injection suggests that insights into the control of blood-borne tumor may also come from further study of this model. Kluwer Academic Publishers, 1990 neuroblastoma nationallicence metastasis nationallicence immune response nationallicence nude mice nationallicence beige mice nationallicence beige-nude mice nationallicence Turner William Children's Cancer Research Center, Children's Hospital of Philadelphia, USA aut Chatten Jane Department of Anatomic Pathology, Children's Hospital of Philadelphia, 19104, Philadelphia, PA aut Lampson Lois Center for Neurologic Diseases, Biosciences Research Building, Brigham and Women's Hospital, 02115, Boston, MA, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/2(1990-04-01), 121-132 0167-594X 8:2<121 1990 8 11060 https://doi.org/10.1007/BF00177834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00177834 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turner William Children's Cancer Research Center, Children's Hospital of Philadelphia, USA aut NATIONALLICENCE 700 1- Chatten Jane Department of Anatomic Pathology, Children's Hospital of Philadelphia, 19104, Philadelphia, PA aut NATIONALLICENCE 700 1- Lampson Lois Center for Neurologic Diseases, Biosciences Research Building, Brigham and Women's Hospital, 02115, Boston, MA, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/2(1990-04-01), 121-132 0167-594X 8:2<121 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer