caa a22 4500 465735789 CHVBK 20180323111754.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00177832 doi (NATIONALLICENCE)springer-10.1007/BF00177832 Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique [Elektronische Daten] [A. Bowles Jr., Cooley Pantazis, William Wansley, Marshall Allen] This report describes a new fluorescent microcarrier cytostasis assay. Human glioma cell lines and primary cultures were attached to microcarrier tissue culture beads and treated with various chemotherapeutic drugs. After treatment, the cells were labelled with two vital fluorescent dyes in order to measure cellular viability. The uptake of hydroethidine and Hoechst 33342 was evaluated alone and in combination as probes for determining metabolic activity and cellular proliferation. Hydroethidine was found to be superior when compared to trypan blue and tritiated thymidine. The use of the microcarrier technique allows for the direct cellular measurement of fluorescence without the need of extensive extraction procedures. The fluorescent assay is a sensitive, rapid and an effective way to screen for potential antiproliferative compounds. Kluwer Academic Publishers, 1990 microassay nationallicence glioma nationallicence cytodex microcarriers nationallicence hydroethidine nationallicence Bowles Jr. A. Department of Surgery, Section of Neurosurgery, Medical College of Georgia, 30912, Augusta, GA, USA aut Pantazis Cooley Department of Pathology, Section of Anatomic Pathology, Medical College of Georgia, 30912, Augusta, GA, USA aut Wansley William Department of Pathology, Section of Anatomic Pathology, Medical College of Georgia, 30912, Augusta, GA, USA aut Allen Marshall Department of Surgery, Section of Neurosurgery, Medical College of Georgia, 30912, Augusta, GA, USA aut Journal of Neuro-Oncology Kluwer Academic Publishers 8/2(1990-04-01), 103-112 0167-594X 8:2<103 1990 8 11060 https://doi.org/10.1007/BF00177832 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00177832 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bowles Jr A. Department of Surgery, Section of Neurosurgery, Medical College of Georgia, 30912, Augusta, GA, USA aut NATIONALLICENCE 700 1- Pantazis Cooley Department of Pathology, Section of Anatomic Pathology, Medical College of Georgia, 30912, Augusta, GA, USA aut NATIONALLICENCE 700 1- Wansley William Department of Pathology, Section of Anatomic Pathology, Medical College of Georgia, 30912, Augusta, GA, USA aut NATIONALLICENCE 700 1- Allen Marshall Department of Surgery, Section of Neurosurgery, Medical College of Georgia, 30912, Augusta, GA, USA aut NATIONALLICENCE 773 0- Journal of Neuro-Oncology Kluwer Academic Publishers 8/2(1990-04-01), 103-112 0167-594X 8:2<103 1990 8 11060 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer