caa a22 4500 465736890 CHVBK 20180323111757.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF02245738 doi (NATIONALLICENCE)springer-10.1007/BF02245738 Close correlation between behavioural response and binding in vivo for inhibitors of the rolipram-sensitive phosphodiesterase [Elektronische Daten] [Ralph Schmiechen, Herbert Schneider, Helmut Wachtel] The antidepressant rolipram interacts in vitro with a binding site in brain tissue labeled by3H-rolipram. A3H-rolipram binding assay was employed in vivo to compare the affinity of rolipram-related compounds and reference phosphodiesterase (PDE) inhibitors with their potency in behavioural measures for potential antidepressant property. In two species, mice and rats, the potency of a number of compounds to antagonise reserpine-induced hypothermia (mice) and to induce head twitches (rats) was determined, as well as their potency to displace3H-rolipram from forebrain binding sites in vivo. The treatment schedules for the two series of experiments were identical. Significant correlations between pharmacological effects and displacement of3H-rolipram binding in vivo were observed in both species. Since the reference PDE inhibitors closely fit into the binding-pharmacological activity relationship, the PDE inhibitory properties of the substances involved are discussed. Springer-Verlag, 1990 Rolipram nationallicence Antidepressant nationallicence In vivo binding nationallicence Hypothermia nationallicence Head twitches nationallicence Schmiechen Ralph Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut Schneider Herbert Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut Wachtel Helmut Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut Psychopharmacology Springer-Verlag 102/1(1990-09-01), 17-20 0033-3158 102:1<17 1990 102 213 https://doi.org/10.1007/BF02245738 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02245738 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmiechen Ralph Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut NATIONALLICENCE 700 1- Schneider Herbert Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut NATIONALLICENCE 700 1- Wachtel Helmut Department of Neuropsychopharmacology, Research Laboratories of Schering AG, P.O. Box 650311, D-1000, Berlin aut NATIONALLICENCE 773 0- Psychopharmacology Springer-Verlag 102/1(1990-09-01), 17-20 0033-3158 102:1<17 1990 102 213 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer