caa a22 4500 465743218 CHVBK 20180323111813.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02697355 doi (NATIONALLICENCE)springer-10.1007/BF02697355 Choi Jeong-Woo Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 08855, New Brunswick, NJ aut Production of berberine in immobilized plant cell culture of Thalictrum rugosum [Elektronische Daten] [Jeong-Woo Choi] Thalictrum rugosum cells were immobilized in calcium alginate, where they continued to live with their biological activity. The immobilized living cells performed the production of berberine in both shake flasks and an airlift bioreactor. Berberine formation was growth associative and most of the berberine produced was stored intracellularly. Rapid hydrolysis of sucrose and preference of glucose over fructose during the growth stage was observed. Phosphate-deficient media increased berberine production and prevented the dissolution of alginate beads. The behavior of immobilized cells grown in an airlift reactor was compared with that of the corresponding shake flask culture with respect to growth and berberine production. The rate of cell growth and berberine production in an airlift reactor operation was higher than those in packedcolumn reactor operation due to a better oxygen transfer. Korean Institute of Chemical Engineering, 1990 Korean Journal of Chemical Engineering Springer-Verlag 7/3(1990-07-01), 226-232 0256-1115 7:3<226 1990 7 11814 https://doi.org/10.1007/BF02697355 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02697355 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Choi Jeong-Woo Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 08855, New Brunswick, NJ aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer-Verlag 7/3(1990-07-01), 226-232 0256-1115 7:3<226 1990 7 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer