caa a22 4500 46574446X CHVBK 20180323111816.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02240413 doi (NATIONALLICENCE)springer-10.1007/BF02240413 Antigenic characterisation of virus isolates from vaccinated dogs dying of rabies [Elektronische Daten] [H. Aghomo, C. Rupprecht] Caracterisation Antigenique D'Isolats Viraux Obtenus A Partir De Cas Mortels De Rage Canine, Malgre La Vaccination Summary: Four rabies virus isolates from dogs that succumbed to rabies infection in Nigeria within one year of anti-rabies vaccination were characterised by monoclonal antibodies (MAbs). The samples were screened for rabies and rabies-related viral antigens by the indirect fluorescent antibody test, performed with MAb 502-2, which recognises the nucleocapsid (NC) protein of all known Lyssaviruses and with MAb 422-5 which identifies African rabiesrelated viruses. All four canine virus isolates displayed positive fluorescence with MAb 502-2 and were negative with MAb 422-5. In the anti-NC MAb characterisation with a panel of 34 additional MAbs, all isolates displayed positive staining with 32 of the MAbs, were negative with MAb 102-27 and all displayed poor immunofluorescence with MAb 377-7. On the basis of reactivity with a panel of 40 anti-glycoprotein (G) MAbs the isolates were separated into four distinct viral subtypes. None of these canine isolates was identified as the common attenuated Flury LEP rabies strain used for domestic animal vaccination and none resembled other previously characterised rabies viruses from Nigeria. Centre for Tropical Veterinary Medicine, 1990 Aghomo H. The Wistar Institute of Anatomy and Biology, 3601 Spruce Street, 19104, Philadelphia, PA, USA aut Rupprecht C. The Wistar Institute of Anatomy and Biology, 3601 Spruce Street, 19104, Philadelphia, PA, USA aut Tropical Animal Health and Production Kluwer Academic Publishers-Human Sciences Press 22/4(1990-12-01), 275-280 0049-4747 22:4<275 1990 22 11250 https://doi.org/10.1007/BF02240413 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02240413 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aghomo H. The Wistar Institute of Anatomy and Biology, 3601 Spruce Street, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Rupprecht C. The Wistar Institute of Anatomy and Biology, 3601 Spruce Street, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 773 0- Tropical Animal Health and Production Kluwer Academic Publishers-Human Sciences Press 22/4(1990-12-01), 275-280 0049-4747 22:4<275 1990 22 11250 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer