caa a22 4500 465744818 CHVBK 20180323111817.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01808057 doi (NATIONALLICENCE)springer-10.1007/BF01808057 Adsorption of 5′-adenosine monophosphate onto precipitated calcium phosphate: Effects of inorganic polyphosphates and carbamyl phosphate [Elektronische Daten] [Marcelo Hermes-Lima, Ana Tessis, Adalberto Vieyra] In this paper it is shown that the adsorption of 5′-adenosine monophosphate (5′-AMP) onto precipitated calcium phosphate exhibits a sigmoidal profile as revealed by isotherms at 45 °C. This result indicates a cooperative behavior in the adsorption of 5′-AMP. The relationship between adsorption capacity and surface area of the sedimented matrix may be interpreted as an indication that there is a monolayer of the adsorbed nucleotide on the solid surface. The pH dependence of adsorption suggests that the negatively charged phosphoryl group of 5′-AMP interacts with a positively charged site (possibly Ca2+) on the matrix surface. The adsorption of the nucleotide is markedly decreased at pH values above 8.0. The Dixon-like plot of the effect of pH suggests an inhibitory role of hydroxyl ions in the adsorption of 5′-AMP. At pH 7.5, other anions such as pyrophosphate, tripolyphosphate and carbamyl phosphate also inhibit the adsorption of the nucleotide, probably by interacting with its adsorption site. We suggest that these phosphorylated molecules could have played a role in chemical evolution by modulating the amount of nucleotides adsorbed onto mineral surfaces. The significance of these phenomena in chemical evolution is discussed. Kluwer Academic Publishers, 1990 Hermes-Lima Marcelo Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut Tessis Ana Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut Vieyra Adalberto Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut Origins of life and evolution of the biosphere Kluwer Academic Publishers 20/1(1990-01-01), 27-41 0169-6149 20:1<27 1990 20 11084 https://doi.org/10.1007/BF01808057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01808057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hermes-Lima Marcelo Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut NATIONALLICENCE 700 1- Tessis Ana Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut NATIONALLICENCE 700 1- Vieyra Adalberto Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21910, Rio de Janeiro, RJ, Brasil aut NATIONALLICENCE 773 0- Origins of life and evolution of the biosphere Kluwer Academic Publishers 20/1(1990-01-01), 27-41 0169-6149 20:1<27 1990 20 11084 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465744818 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer