caa a22 4500 465745202 CHVBK 20180323111818.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02624723 doi (NATIONALLICENCE)springer-10.1007/BF02624723 Cell features in pancreas of prediabetic and diabetic rabbits after wirsung duct ligation. Histochemical and histoenzymatic studies [Elektronische Daten] [Jacqueline Catala, Pierre Hadjiisky, Roger Bonnafous, Françoise Fichaux] Summary: Kinetic studies of the histochemical and histoenzymatic behavior of rabbit pancreatic parenchymas were performed 5, 30 and 90 days after Wirsung duct ligation. In control pancreas, some enzyme activities (EA) were more prominent in Langerhans islets [glucose-6-phosphatase, glucose-6-phosphate dehydrogenase (DH), isocitrate DH, glycerol-3-phosphate DH, NADPH DH], others were strongly marked in acini and ducts (alkaline phosphatase, β-glucuronidase, acid esterase aryl-sulfatase). Histochemical and enzyme abnormalities observed in experimental rabbits reflect the post-ligation degenerative and reactive processes in both exocrine and endocrine pancreas: (1) the decrease in Krebs cycle and pentose pathway linked EA and the increased lysosomal and acid phosphatase EA reflect early (day 5) degeneration and necrosis of islets and acini (day 30); (2) proliferative processes in developed ductal epithelia are shown by an increase in both glycolytic and lysosomal EA (days 30 and 90); (3) connective tissue neogenesis and interstitial fibrosis occurred as shown by activated β-glucuronidase, aryl-sulfatase, alkaline phosphatase and increased ribonucleoproteins and glycoaminoglycans contents (day 30); (4) on day 90, the neoformed cell clusters presenting glucose-6-phosphatase positivity (B-cell marker) are seen in the pancreas remnant. At the same time, blood insulin level increases correlated with a decrease of hyperglycemia. Casa Editrice «il Ponte», 1990 Diabetes nationallicence Glycemia nationallicence Histoenzymology nationallicence Hydrolases nationallicence Insulin nationallicence Oxidoreductases nationallicence Pancreatic duct ligation nationallicence Pancreas nationallicence Rabbit nationallicence Catala Jacqueline Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut Hadjiisky Pierre Centre de Recherche des Maladies Cardiovasculaires Pitié-Salpétrières, Paris, France aut Bonnafous Roger Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut Fichaux Françoise Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut Acta diabetologia latina Springer-Verlag 27/1(1990-03-01), 59-69 0001-5563 27:1<59 1990 27 592 https://doi.org/10.1007/BF02624723 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02624723 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Catala Jacqueline Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut NATIONALLICENCE 700 1- Hadjiisky Pierre Centre de Recherche des Maladies Cardiovasculaires Pitié-Salpétrières, Paris, France aut NATIONALLICENCE 700 1- Bonnafous Roger Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut NATIONALLICENCE 700 1- Fichaux Françoise Laboratoire de Physiologie de la Digestion et de la Nutrition, Institut de Physiologie, Université Paul Sabatier, Toulouse aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/1(1990-03-01), 59-69 0001-5563 27:1<59 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer