caa a22 4500 465745261 CHVBK 20180323111818.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02624721 doi (NATIONALLICENCE)springer-10.1007/BF02624721 Ketone body kinetics in vivo using simultaneous administration of acetoacetate and 3-hydroxybutyrate labelled with stable isotopes [Elektronische Daten] [Angelo Avogaro, Romano Nosadini, Dennis Bier, Claudio Cobelli, Gianna Toffolo, Alessandro Doria, Anna Valerio, Howard Christopherson] Summary: Isotope dilution studies of ketone body (KB) turnover have usually been performed using a single14C tracer and the so called ‘combined KB specific activity'. By definition, this approach does not allow to evaluate the individual kinetics of acetoacetate (AcAc) and 3-hydroxybutyrate (R-BHB) which is feasible only using the separate administration of14C tracer AcAc and R-BHB. In the present study we followed a different approach using the simultaneous administrationin vivo of [1,2,13C2] AcAc and m [1,2,3,413C4] R-BHB which allows to evaluate the individual kinetics of the two KB in the some study, thus minimizing the magnitude of blood sampling and the potential changes in the metabolic conditions of each subject. The four isotopic13C/12C KB ratios of AcAc and R-BHB tracer and tracee blood concentrations along with the fluorimetric measurement of12C concentrations were determined in each blood sample. Using compartmental analysis following single dose bolus injection the production rate of KB was 206 ± 57 µmol/min/1.73 m2 (mean ± SD). The turnover rate of KB using noncompartmental analysis, during continuous infusion in a separate study was 294 ± 41. The plasma clearance rates of AcAc and R-BHB were 1966 ± 502 and 1443 ± ml/min/1.73 m2, respectively. The mean residence time was 17 ± 3 min and the total distribution volume 20 ± 9.7 1/m2. We conclude that: (1) stable isotope tracer infusion allows the contemporaryin vivo administration of the two KB and the simultaneous assessment of individual AcAc and R-BHB kinetics; (2) the estimated compartmental and noncompartmental parameters of KB turnover were similar to those observed in normal overnight fasting subjects following separate radioactive tracer injections. Casa Editrice «il Ponte», 1990 Acetoacetate nationallicence 3-Hydroxybutyrate nationallicence Ketone bodies nationallicence Mass spectrometry nationallicence Modelling analysis nationallicence Stable isotopes nationallicence Turnover nationallicence Avogaro Angelo Cattedra di Malattie del Ricambio, Università di Padova, Padova aut Nosadini Romano Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut Bier Dennis Metabolism Division, Washington University, St. Louis, MO, U.S.A. aut Cobelli Claudio Istituto di Elettrotecnica e Elettronica, Università di Padova, Padova, Italy aut Toffolo Gianna Istituto di Elettrotecnica e Elettronica, Università di Padova, Padova, Italy aut Doria Alessandro Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut Valerio Anna Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut Christopherson Howard Metabolism Division, Washington University, St. Louis, MO, U.S.A. aut Acta diabetologia latina Springer-Verlag 27/1(1990-03-01), 41-51 0001-5563 27:1<41 1990 27 592 https://doi.org/10.1007/BF02624721 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02624721 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Avogaro Angelo Cattedra di Malattie del Ricambio, Università di Padova, Padova aut NATIONALLICENCE 700 1- Nosadini Romano Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut NATIONALLICENCE 700 1- Bier Dennis Metabolism Division, Washington University, St. Louis, MO, U.S.A aut NATIONALLICENCE 700 1- Cobelli Claudio Istituto di Elettrotecnica e Elettronica, Università di Padova, Padova, Italy aut NATIONALLICENCE 700 1- Toffolo Gianna Istituto di Elettrotecnica e Elettronica, Università di Padova, Padova, Italy aut NATIONALLICENCE 700 1- Doria Alessandro Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut NATIONALLICENCE 700 1- Valerio Anna Cattedra di Patologia Medica, Università di Padova, Padova, Italy aut NATIONALLICENCE 700 1- Christopherson Howard Metabolism Division, Washington University, St. Louis, MO, U.S.A aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/1(1990-03-01), 41-51 0001-5563 27:1<41 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer