caa a22 4500 465745296 CHVBK 20180323111819.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF02581331 doi (NATIONALLICENCE)springer-10.1007/BF02581331 Islet isolation assessment in man and large animals [Elektronische Daten] [Camillo Ricordi, Derek Gray, Bernhard Hering, Dixon Kaufman, Garth Warnock, Norman Kneteman, Stephen Lake, Nicholas London, Carlo Socci, Rodolfo Alejandro, Yijun Zeng, David Scharp, Giorgio Viviani, Luca Falqui, Andreas Tzakis, Reinhard Bretzel, Konrad Federlin, Guido Pozza, Roger James, Ray Rajotte, Valerio Carlo, Peter Morris, David Sutherland, Thomas Starzl, Daniel Mintz, Paul Lacy] Summary: Recent progress in islet isolation from the pancreas of large mammals including man, accentuated the need for the development of precise and reproducible techniques to assess islet yield. In this report both quantitative and qualitative criteria for islet isolation assessment were discussed, the main topics being the determination of number, volume, purity, morphologic integrity andin vitro andin vivo function tests of the final islet preparations. It has been recommended that dithizone should be used as a specific stain for immediate detection of islet tissue making it possible to estimate both the total number of islets (dividing them into classes of 50 µ diameter range increments) and the purity of the final preparation. Appropriate morphological assessment should include confirmation of islet identification, assessment of the morphological integrity and of the purity of the islet preparation. The use of fluorometric inclusion and exclusion dyes together have been suggested as a viability assay to simultaneously quantitate the proportion of cells that are intact or damaged. Perifusion of islets with glucose provides a dynamic profile of glucose-mediated insulin release and of the ability of the cells to down regulate insulin secretion after the glycemic challenge is interrupted. Although perifusion data provides a useful guide to islet viability the quantity and kinetics of insulin release do not necessarily predict islet performance after implantation. Therefore, the ultimate test of islet viability is their function after transplantation into a diabetic recipient. For this reason,in vivo models of transplantation of an aliquot of the final islet preparation into diabetic nude (athymic) rodents have been suggested. We hope that these general guidelines will be of assistance to standardize the assessment of islet isolations, making it possible to better interpret and compare procedures from different centers. Casa Editrice il Ponte, 1990 Assessment nationallicence Islet nationallicence Isolation nationallicence Ricordi Camillo Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut Gray Derek University of Oxford, Oxford, U.K. aut Hering Bernhard Justus Liebig University, Giessen, FRG aut Kaufman Dixon University of Minnesota, Minneapolis, Minnesota, U.S.A. aut Warnock Garth University of Alberta, Edmonton, Alberta, Canada aut Kneteman Norman University of Alberta, Edmonton, Alberta, Canada aut Lake Stephen University of Leichester, Leichester, U.K. aut London Nicholas University of Leichester, Leichester, U.K. aut Socci Carlo Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut Alejandro Rodolfo University of Miami, Miami, Florida, U.S.A. aut Zeng Yijun Capital Medical College, Beijing, China aut Scharp David Washington University, St. Louis, Missouri, U.S.A. aut Viviani Giorgio Università degli Studi di Genova, Genova, Italy aut Falqui Luca Washington University, St. Louis, Missouri, U.S.A. aut Tzakis Andreas University of Pittsburgh, Pittsburgh, U.S.A. aut Bretzel Reinhard Justus Liebig University, Giessen, FRG aut Federlin Konrad Justus Liebig University, Giessen, FRG aut Pozza Guido Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut James Roger University of Leichester, Leichester, U.K. aut Rajotte Ray University of Alberta, Edmonton, Alberta, Canada aut Carlo Valerio Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut Morris Peter University of Alberta, Edmonton, Alberta, Canada aut Sutherland David University of Minnesota, Minneapolis, Minnesota, U.S.A. aut Starzl Thomas University of Pittsburgh, Pittsburgh, U.S.A. aut Mintz Daniel University of Miami, Miami, Florida, U.S.A. aut Lacy Paul Washington University, St. Louis, Missouri, U.S.A. aut Acta diabetologia latina Springer-Verlag 27/3(1990-07-01), 185-195 0001-5563 27:3<185 1990 27 592 https://doi.org/10.1007/BF02581331 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02581331 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ricordi Camillo Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 700 1- Gray Derek University of Oxford, Oxford, U.K aut NATIONALLICENCE 700 1- Hering Bernhard Justus Liebig University, Giessen, FRG aut NATIONALLICENCE 700 1- Kaufman Dixon University of Minnesota, Minneapolis, Minnesota, U.S.A aut NATIONALLICENCE 700 1- Warnock Garth University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Kneteman Norman University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Lake Stephen University of Leichester, Leichester, U.K aut NATIONALLICENCE 700 1- London Nicholas University of Leichester, Leichester, U.K aut NATIONALLICENCE 700 1- Socci Carlo Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 700 1- Alejandro Rodolfo University of Miami, Miami, Florida, U.S.A aut NATIONALLICENCE 700 1- Zeng Yijun Capital Medical College, Beijing, China aut NATIONALLICENCE 700 1- Scharp David Washington University, St. Louis, Missouri, U.S.A aut NATIONALLICENCE 700 1- Viviani Giorgio Università degli Studi di Genova, Genova, Italy aut NATIONALLICENCE 700 1- Falqui Luca Washington University, St. Louis, Missouri, U.S.A aut NATIONALLICENCE 700 1- Tzakis Andreas University of Pittsburgh, Pittsburgh, U.S.A aut NATIONALLICENCE 700 1- Bretzel Reinhard Justus Liebig University, Giessen, FRG aut NATIONALLICENCE 700 1- Federlin Konrad Justus Liebig University, Giessen, FRG aut NATIONALLICENCE 700 1- Pozza Guido Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 700 1- James Roger University of Leichester, Leichester, U.K aut NATIONALLICENCE 700 1- Rajotte Ray University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Carlo Valerio Istituto H. San Raffaele, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 700 1- Morris Peter University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Sutherland David University of Minnesota, Minneapolis, Minnesota, U.S.A aut NATIONALLICENCE 700 1- Starzl Thomas University of Pittsburgh, Pittsburgh, U.S.A aut NATIONALLICENCE 700 1- Mintz Daniel University of Miami, Miami, Florida, U.S.A aut NATIONALLICENCE 700 1- Lacy Paul Washington University, St. Louis, Missouri, U.S.A aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/3(1990-07-01), 185-195 0001-5563 27:3<185 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer