caa a22 4500 465745415 CHVBK 20180323111819.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02580934 doi (NATIONALLICENCE)springer-10.1007/BF02580934 Serum spermidine oxidase activity in patients with insulin-dependent diabetes mellitus and microvascular complications [Elektronische Daten] [Giuseppe Seghieri, Alfredo Gironi, Margherita Niccolai, Piero Mammini, Lorenzo Alviggi, Lamberto De Giorgio, Paolo Caselli, Giancarlo Bartolomei] Summary: Metabolism of polyamines (spermidine and spermine) is known to be strictly related to the growth processes of eukaryotic cells. Since cell replication processes appear altered in insulin-dependent diabetes mellitus (IDDM), especially when associated with its microvascular complications, the aim of this study was measuring serum spermidine oxidase activity (SOA), a key enzyme in the metabolic pathway of polyamines, in 47 patients with IDDM as compared with 63 healthy control subjects matched for age and sex. Mean SOA levels±SD were significantly lower in IDDM patients (177.4±57.2 µkat/l) than in controls (247.6±68.1 µkat/l; p<0.001), being SOA inversely related with daily insulin dose. SOA was moreover significantly higher (but similar to controls) in the group with increased urinary albumin excretion rate (AER persistently >20 µg/min); (n=17; 213.1±62.6 µkat/l) in comparison with normoalbuminuric subjects (n=30; 156.6±43.5 µkat/l; F=21.78; p=0.0001). SOA was correlated with AER (r=0.45; p=0.001), independently of age, duration of disease, serum creatinine, body weight, blood pressure and metabolic control, as shown by a multiple regression analysis model (p=0.003). Presence of background retinopathy was not associated with modified levels of SOA, which was conversely higher, although not significantly, in the patients with proliferative retinal lesions. In conclusion serum SOA is deeply altered in IDDM patients, being markedly reduced in the whole group of patients and conversely independently increased up to the mean values of controls in presence of increased AER or advanced retinopathy. Casa Editrice «il Ponte», 1990 Insulin-dependent diabetes mellitus nationallicence Microangiopathy nationallicence Polyamine metabolism nationallicence Retinopathy nationallicence Spermidine oxidase nationallicence Urinary albumin excretion rate nationallicence Seghieri Giuseppe Sezione di Diabetologia, Italy aut Gironi Alfredo Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut Niccolai Margherita Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut Mammini Piero Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut Alviggi Lorenzo Sezione di Diabetologia, Italy aut De Giorgio Lamberto Sezione di Diabetologia, Italy aut Caselli Paolo Sezione di Diabetologia, Italy aut Bartolomei Giancarlo Sezione di Diabetologia, Italy aut Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 303-308 0001-5563 27:4<303 1990 27 592 https://doi.org/10.1007/BF02580934 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02580934 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seghieri Giuseppe Sezione di Diabetologia, Italy aut NATIONALLICENCE 700 1- Gironi Alfredo Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut NATIONALLICENCE 700 1- Niccolai Margherita Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut NATIONALLICENCE 700 1- Mammini Piero Istituto di Chimica Clinica, Spedali Riuniti, Pistoia, Italy aut NATIONALLICENCE 700 1- Alviggi Lorenzo Sezione di Diabetologia, Italy aut NATIONALLICENCE 700 1- De Giorgio Lamberto Sezione di Diabetologia, Italy aut NATIONALLICENCE 700 1- Caselli Paolo Sezione di Diabetologia, Italy aut NATIONALLICENCE 700 1- Bartolomei Giancarlo Sezione di Diabetologia, Italy aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 303-308 0001-5563 27:4<303 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer