caa a22 4500 465745482 CHVBK 20180323111819.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02580936 doi (NATIONALLICENCE)springer-10.1007/BF02580936 Influence of estrogen-progesterone sequential administration on pancreas cytology. Serum insulin and metabolic adjustments in female dogs [Elektronische Daten] [Aurora Renauld, Rita Sverdlik, Alicia Agüero, Ricardo Pérez] Summary: In bitches in anestrus, artificial endometrial sex cycles were induced. Estrus was induced by 17β-estradiol benzoate administration; matched untreated and vehicle-treated controls were studied. Early metadiestrus (6th day after appearance of metestrum cells in vaginal smears) was induced by the sequential administration of 17β-estradiol benzoate and progesterone: matched studies with only one hormone or vehicles were also carried out. In different groups of animals, blood sugar (BS), serum immunoreactive insulin (IRI) and serum free fatty acids (FFA) in the basal conditions and during glucose and insulin tests were studied. Insulin was immunocytolocalized in sections of pancreas from a part of these animals. Size and insulin content in Langerhans islets were measured by morphometric and cytospectrophotometric computerized analysis. Extrapancreatic factors — space of distribution, t½ in blood stream — regulating serum IRI and BS levels were calculated. The hypoglycemic effect of insulin was enhanced by estrogenization, together with insulin accumulation in Langerhans islets. Progesterone treatment caused mild insulin resistance together with depletion of pancreatic insulin stores in the long run. Glucose tolerance of progesterone-injected bitches was improved after estrogen priming with greater space of distribution of glucose. Furthermore, a high basal serum FFA levels in bitches receiving the hormone sequence was observed. We may therefore conclude that the metabolic and endocrine changes induced in bitches by artificial sex cycles converge towards excellent BS homeostasis leads to the replenishing of pancreatic insulin stores, so that estrogen-progesterone administration in sequence appears to be, in this experimental condition, non-diabetogenic. Casa Editrice «il Ponte», 1990 Artificial estrus cycle nationallicence β-estradiol nationallicence Blood sugar nationallicence Glucose test nationallicence Insulin test nationallicence Progesterone nationallicence Serum insulin nationallicence Serum free fatty acids nationallicence Renauld Aurora Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut Sverdlik Rita Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut Agüero Alicia Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut Pérez Ricardo Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 315-327 0001-5563 27:4<315 1990 27 592 https://doi.org/10.1007/BF02580936 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02580936 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renauld Aurora Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut NATIONALLICENCE 700 1- Sverdlik Rita Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut NATIONALLICENCE 700 1- Agüero Alicia Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut NATIONALLICENCE 700 1- Pérez Ricardo Departamento de Fisiología and Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 315-327 0001-5563 27:4<315 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer