caa a22 4500 465745504 CHVBK 20180323111819.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF02580940 doi (NATIONALLICENCE)springer-10.1007/BF02580940 Colorimetric versus radioimmunological measurement of glycated and non-glycated serum albumin after affinity chromatography [Elektronische Daten] [Lorella Cruschelli, Aldo Clerico, Giuseppe Penno, Renzo Navalesi, Ottavio Giampietro] Summary: Affinity chromatography bym-aminophenylboronic acid has been proposed for routine measurement of glycated albumin. We assayed glycated and non-glycated fractions of serum albumin (HSA) eluted by affinity chromatography columns by both a specific RIA method for the human serum albumin (HSA) and by a colorimetric method. Sixteen diabetic patients presented a significantly higher percentage of glycated-HSA than 7 control subjects with both methods, and a strong correlation was found between the values obtained with the two methods. RIA was able to dectect a significant concentration of glycated-HSA in all normal subjects, while the colorimetric method was not. The accuracy of separation between the glycated and non-glycated fractions of albumin was tested using [14C]glucose as tracer. When [14C]glycated-HSA purified by Sephadex G25 filtration was chromatographed using them-aminophenylboronic acid, only 5.3% of the total14C-radioactivity present in the solution was recovered in the bound fraction, while 44.0% was eluted in non-protein-bound fraction and 54.7% was retained in the column. Our findings confirm that affinity chromatography bym-aminophenylboronic acid can be a useful tool in the monitoring of short glycemic control of diabetic patients. Our data also indicate that the affinity chromatography withm-aminophenylboronic acid does not accurately discriminate between glycated and non-glycated fraction of HSA. Casa Editrice «il Ponte», 1990 Affinity chromatography nationallicence m-Aminophenylboronic acid nationallicence Diabetes mellitus nationallicence Glycated proteins nationallicence Glycation nationallicence Cruschelli Lorella Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut Clerico Aldo Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut Penno Giuseppe Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut Navalesi Renzo Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut Giampietro Ottavio Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 349-356 0001-5563 27:4<349 1990 27 592 https://doi.org/10.1007/BF02580940 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02580940 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cruschelli Lorella Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Clerico Aldo Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Penno Giuseppe Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Navalesi Renzo Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut NATIONALLICENCE 700 1- Giampietro Ottavio Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Istituto di Fisiologia Clinica del C.N.R., Università degli Studi di Pisa, Pisa, Italy aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/4(1990-10-01), 349-356 0001-5563 27:4<349 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer