caa a22 4500 465745571 CHVBK 20180323111819.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF02581285 doi (NATIONALLICENCE)springer-10.1007/BF02581285 Whole blood L-lactate assay by a new differential pH method: Application to metabolic investigations [Elektronische Daten] [Livio Luzi, Marilena Ripamonti, Claudio Marconi, Elisabetta Cazzola, Andrea Mosca] Summary: We propose a new quantitative method for L-lactate assay in whole blood, based on the measurement of pH variation caused by specific and irreversible oxidation of L-lactate to pyruvate in the presence of an electron acceptor (hexacyanoferrate) and of the enzyme cytochrome b2 (EC 1.1.2.3.). No sample pretreatment is needed; the method is simple and fast (1.5 min/analysis) and requires 10 µl whole blood per assay. Linearity is confirmed up to 20 mmol/l L-lactate. Within-day and between-day variability was (as C.V.) 3.6% and 8.1% for blood lactate 1.3 and 1.0 mmol/l, respectively. The results by the present method correlate well with those from two reference methods (test methodvs a lactate sensor based method: r=0.996; test methodvs a spectrophotometric method: r=0.987). An application of the present method to the continuous monitoring of L-lactate in patients after combined kidney and pancreas transplantation, under conditions of euglycemic hyperinsulinemia and hyperglycemic hyperinsulinemia is reported. We conclude that the method is simple and reproducible and can be employed to measure whole blood lactate concentration continuously both in clinical protocols and in basic research. Casa Editrica «il Ponte», 1990 Diabetes mellitus nationallicence Differential pH nationallicence Glucose clamp nationallicence Insulin clamp nationallicence Lactate nationallicence Transplantation nationallicence Luzi Livio Divisione di Medicina I, Istituto Scientifico H.S. Raffaele, Via Olgettina, 60, 20132, Milano, Italy aut Ripamonti Marilena I.T.B.A., C.N.R., Milano aut Marconi Claudio I.T.B.A., C.N.R., Milano aut Cazzola Elisabetta Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Milano, Italy aut Mosca Andrea Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Milano, Italy aut Acta diabetologia latina Springer-Verlag 27/2(1990-04-01), 129-138 0001-5563 27:2<129 1990 27 592 https://doi.org/10.1007/BF02581285 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02581285 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luzi Livio Divisione di Medicina I, Istituto Scientifico H.S. Raffaele, Via Olgettina, 60, 20132, Milano, Italy aut NATIONALLICENCE 700 1- Ripamonti Marilena I.T.B.A., C.N.R., Milano aut NATIONALLICENCE 700 1- Marconi Claudio I.T.B.A., C.N.R., Milano aut NATIONALLICENCE 700 1- Cazzola Elisabetta Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 700 1- Mosca Andrea Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Milano, Italy aut NATIONALLICENCE 773 0- Acta diabetologia latina Springer-Verlag 27/2(1990-04-01), 129-138 0001-5563 27:2<129 1990 27 592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer