caa a22 4500 465746144 CHVBK 20180323111821.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00332260 doi (NATIONALLICENCE)springer-10.1007/BF00332260 Immunoaffinity for IgG antibodies of protein A labelled with 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid [Elektronische Daten] [A. Ius, L. Ferrara, G. Meroni, M. Bacigalupo] Summary: Protein A was covalently labelled with 4,7-bis-(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid in several molar ratios. The binding affinity for IgG and the delayed fluorescence of the corresponding complexes with Eu(III) were determined for each labelled compound. The immunoaffinity decreased slowly with increase in ratio of the label. A better immunoaffinity/labelling ratio was obtained by coupling protein A to the chelate via bovine serum albumin. A suitable ratio of chelating agent to protein A for producing a universal marker to be used in time-resolved fluoroimmunoassays can be determined. Springer-Verlag, 1990 Ius A. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut Ferrara L. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut Meroni G. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut Bacigalupo M. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 336/3(1990-01-01), 235-237 0937-0633 336:3<235 1990 336 216 https://doi.org/10.1007/BF00332260 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00332260 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ius A. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut NATIONALLICENCE 700 1- Ferrara L. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut NATIONALLICENCE 700 1- Meroni G. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut NATIONALLICENCE 700 1- Bacigalupo M. Istituto Chimica Ormoni, C. N. R., via Mario Bianco 9, I-20131, Milano, Italy aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 336/3(1990-01-01), 235-237 0937-0633 336:3<235 1990 336 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer