caa a22 4500 465747523 CHVBK 20180323111824.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00323162 doi (NATIONALLICENCE)springer-10.1007/BF00323162 Biosampling for metal speciation [Elektronische Daten] I. Determination of chromate in the presence of Cr(III) by separation with cell membranes and GFAAS [B. Neidhart, S. Herwald, Ch. Lippmann, B. Straka-Emden] Summary: Human erythrocytes (ery's) under physiological conditions are capable of a selective uptake of dissolved chromates beside Cr(III) from aqueous solutions. For sampling the mechanical stability of the red blood cells is increased by immobilization in Ca-alginate beads without loss of the biochemical activity against Cr(VI). Radiotracer studies were performed in order to determine the influence of temperature, pH, and concentration and solubility of chromates on the accumulation, which follows a Michaelis-Menten kinetics. After sampling the ery's are separated from the gel and introduced into a multiple-step clean-up procedure preceding a GFAAS measurement of chromium. The direct determination of chromium in erythrocytes (and comparable matrices) by GFAAS is supported by the use of oxygen as alternate gas during the thermal decomposition of the samples, leading to advantages in routine analysis. The statistical parameters of this method are critically reviewed. The combination of biosampling and GFAAS represents a new way for metal speciation, especially in cases where decomposition of the analyte during standard sampling procedures is likely; this is demonstrated for Cr(III)/Cr(VI) as an example in view of immission measurements of Cr(VI) in airborne particulates. Springer-Verlag, 1990 Neidhart B. Department of Analytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, D-7900, Ulm, Federal Republic of Germany aut Herwald S. Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut Lippmann Ch Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut Straka-Emden B. Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 337/8(1990-01-01), 853-859 0937-0633 337:8<853 1990 337 216 https://doi.org/10.1007/BF00323162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00323162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neidhart B. Department of Analytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, D-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Herwald S. Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lippmann Ch Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Straka-Emden B. Central Scientific Unit Analytical Chemistry, Institute of Occupational Health, Ardeystrasse 67, D-4600, Dortmund 1, Federal Republic of Germany aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 337/8(1990-01-01), 853-859 0937-0633 337:8<853 1990 337 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer