caa a22 4500 465748066 CHVBK 20180323111826.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00322787 doi (NATIONALLICENCE)springer-10.1007/BF00322787 Evaluation of the proteolysis degree with the o-phthalaldehyde/N-acetyl-L-cysteine reagent [Elektronische Daten] [M. Hernández, E. Domingo, R. Camañas, M. Alvarez-Coque] Summary: The o-phthalaldehyde/N-acetyl-L-cysteine (OPA-NAC) reagent is applied to the spectrophotometric evaluation of the proteolytic activity of enzymes. The high stability of the OPA-NAC isoindoles makes a strict control of the time of reaction unnecessary. A mathematical expression is proposed to calculate proteolysis degrees, where the absorbance decrease of the OPA-NAC derivative of the protein itself during the hydrolysis process is taken into account. The method is applied to bovine serum albumine, caseine, lysozyme, lactoglobuline and protamine sulphate as substrates, and pronase, papaine, trypsin and chymotrypsin as enzymes. Springer-Verlag, 1990 Hernández M. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut Domingo E. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut Camañas R. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut Alvarez-Coque M. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/1(1990-01-01), 62-65 0937-0633 338:1<62 1990 338 216 https://doi.org/10.1007/BF00322787 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00322787 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hernández M. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut NATIONALLICENCE 700 1- Domingo E. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut NATIONALLICENCE 700 1- Camañas R. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut NATIONALLICENCE 700 1- Alvarez-Coque M. Departamento de Química Analítica, Facultad de Química, Universitat de Valencia, Burjasot, E-46100, Valencia, Spain aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/1(1990-01-01), 62-65 0937-0633 338:1<62 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer