caa a22 4500 465748295 CHVBK 20180323111826.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00323421 doi (NATIONALLICENCE)springer-10.1007/BF00323421 Fluorimetric enzymatic flow-injection determination of bile acids in human serum [Elektronische Daten] [Antonio Membiela, Fernando Lázaro, M. de Castro, Miguel Valcárcel] Summary: A fast, simple and convenient method for the individual and total determination of bile acids based on the enzymatic oxidation of the analytes and the fluorimetric measurement of a reaction product (the reduced form of the coenzyme, NADH) is proposed. The method allows the determination of the analytes (taurocholic, chenocholic, glycocholic and chenodesoxycholic acid) over the range of 0.3-10.0 μg ml−1, with an r.s.d. smaller than 3.0% in each case. It was applied to the determination of total bile acids in human serum. The simplicity of the method makes it useful for screening clinical samples in order to avoid implementing chromatographic processes on samples with analyte overall contents clearly below pathological limits. Springer-Verlag, 1990 Membiela Antonio Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut Lázaro Fernando Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut de Castro M. Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut Valcárcel Miguel Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/6(1990-01-01), 749-751 0937-0633 338:6<749 1990 338 216 https://doi.org/10.1007/BF00323421 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00323421 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Membiela Antonio Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut NATIONALLICENCE 700 1- Lázaro Fernando Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut NATIONALLICENCE 700 1- de Castro M. Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut NATIONALLICENCE 700 1- Valcárcel Miguel Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004, Córdoba, Spain aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/6(1990-01-01), 749-751 0937-0633 338:6<749 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer