caa a22 4500 465748694 CHVBK 20180323111827.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00322534 doi (NATIONALLICENCE)springer-10.1007/BF00322534 Biological reference materials for assaying human albumin in urine [Elektronische Daten] [Patricia Mueller, Mary MacNeil, Karen Steinberg] Summary: To assess the interlaboratory variation in the results of albumin measurements, we prepared albumin solutions in human urine at various concentrations within the normal range. Since some investigators have reported that albumin is unstable in some human urine samples stored at −20°C, we screened urine samples from 21 persons to identify samples that were stable under these conditions and that had low native albumin content. The urine of two donors met these criteria, and they provided urine, which we prefiltered, sterile-filtered, and spiked with commercially available human serum albumin. The albumin was characterized as pure by a Lowry assay of protein content with National Institute of Standards and Technology bovine serum albumin (standard reference material 926) as the standard and by the appearance of one band on agarose gel electrophoresis. To evaluate the necessity for additional stabilization when urine samples are stored at −20°C, a surfactant was included in one set of materials and not included in another. The materials with surfactant have been evaluated for 10.5 months and those without surfactant for 5 months. The preserved materials showed no significant loss of activity during this period. The unpreserved materials remained stable for 2 months, and then the two higher level materials appeared to loose activity. The negative slope of the highest level of unpreserved material was statistically significant (p=0.01) during this period. In our laboratory, the albumin recovered by enzyme immunoassay was 106.7% and 115.9% in two preserved normal-range materials and 102.2% and 106.3% in similar unpreserved materials. Springer-Verlag, 1990 Mueller Patricia Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut MacNeil Mary Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut Steinberg Karen Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/4(1990-01-01), 543-546 0937-0633 338:4<543 1990 338 216 https://doi.org/10.1007/BF00322534 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00322534 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mueller Patricia Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut NATIONALLICENCE 700 1- MacNeil Mary Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Steinberg Karen Division of Environmental Health Laboratory Sciences, Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 30333, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/4(1990-01-01), 543-546 0937-0633 338:4<543 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer