caa a22 4500 465748783 CHVBK 20180323111828.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00322509 doi (NATIONALLICENCE)springer-10.1007/BF00322509 Certification of bilirubin SRM 916a [Elektronische Daten] [A. Cohen, E. V, B. Coxon, R. Christensen, M. Welch, R. Paule, D. Becker] Summary: The process used to certify Standard Reference Material (SRM) 916a Bilirubin is described. The certification involved the use of various analytical techniques to detect or quantitate impurities, as well as to characterize the SRM itself. Bilirubin (BR) is believed to exist, in human serum, as the IXα isomer. Samples prepared commercially, including this SRM, also contain the IIIα and XIIIα isomers which are believed to be formed during purification. For the SRM, the three isomers were measured by HPLC and TLC. 1H NMR was used to detect and quantitate chloroform in the BR. Biliverdine and mesobilirubin were not detected. Impurities insoluble in chloroform, the residue from the ashing of BR, and volatiles were measured, in addition to non-acidic impurities and impurities more acidic than BR. The absorptivity of BR in chloroform was measured. A pink fluorescent impurity was detected and measured by TLC. From these analyses, a best estimate of the total amount of impurities was determined, and the BR was issued as SRM 916a with a certified purity of 98.3±0.3%. Springer-Verlag, 1990 Cohen A. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut V E. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Coxon B. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Christensen R. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Welch M. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Paule R. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Becker D. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/4(1990-01-01), 426-429 0937-0633 338:4<426 1990 338 216 https://doi.org/10.1007/BF00322509 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00322509 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cohen A. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- V E. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Coxon B. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Christensen R. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Welch M. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Paule R. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 700 1- Becker D. Center for Analytical Chemistry, National Institute of Standards and Technology, 20899, Gaithersburg, MD, USA aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/4(1990-01-01), 426-429 0937-0633 338:4<426 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer