caa a22 4500 465749380 CHVBK 20180323111829.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00324023 doi (NATIONALLICENCE)springer-10.1007/BF00324023 Does the supplementation of metal ions modify the intrinsic distribution of element species in food extracts? [Elektronische Daten] [Holger Reinecke, Lothar Dunemann] Summary: A combination is presented of gel permeation chromatography for the separation of proteins with atomic absorption spectrometry for the detection of protein-bound metals. For the evaluation of the effects resulting from sample spiking with free metal ions, flame AAS in the direct coupling mode was used. Besides sample spiking we also evaluated the effects of adding traces of metals to the eluent. The separation material was TSK HW-55(S), the bed volume was 27 ml. The investigations were carried out on the example of an aqueous soybean flour extract that had been characterized before in view of its intrinsic metal distributions. The supplementation experiments were focused on zinc and nickel, both having essential as well as toxic properties. For both metals a selective enhancement of metal concentrations was observed after sample spiking in distinct fractions due to free binding capacities of certain proteins. It was also possible to detect hydrated ("free”) metal ions. Zinc is bound to the medium molecular weight (MMW) fraction and to the high molecular weight (HMW) fraction, nickel to the HMW fraction only. A depletion of metal ions from the mobile phase was observed in case of the elution of proteins with free binding capacities. Springer-Verlag, 1990 Reinecke Holger Institut für Anorganische und Analytische Chemie, TU Clausthal, Paul-Ernst-Strasse 4, D-3392, Clausthal-Zellerfeld, Federal Republic of Germany aut Dunemann Lothar Institut für Anorganische und Analytische Chemie, TU Clausthal, Paul-Ernst-Strasse 4, D-3392, Clausthal-Zellerfeld, Federal Republic of Germany aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/5(1990-01-01), 630-634 0937-0633 338:5<630 1990 338 216 https://doi.org/10.1007/BF00324023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00324023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reinecke Holger Institut für Anorganische und Analytische Chemie, TU Clausthal, Paul-Ernst-Strasse 4, D-3392, Clausthal-Zellerfeld, Federal Republic of Germany aut NATIONALLICENCE 700 1- Dunemann Lothar Institut für Anorganische und Analytische Chemie, TU Clausthal, Paul-Ernst-Strasse 4, D-3392, Clausthal-Zellerfeld, Federal Republic of Germany aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/5(1990-01-01), 630-634 0937-0633 338:5<630 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer