caa a22 4500 465749623 CHVBK 20180323111830.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00321882 doi (NATIONALLICENCE)springer-10.1007/BF00321882 Enzymatic photometric determination of lactate by reversible reduction of methylene blue [Elektronische Daten] [Anja Rohen, Jörg Krause, Karl Cammann] Summary: A method for lactate determination is described which is based upon oxidation to pyruvate catalyzed by l-lactic dehydrogenase (LDH) from baker's yeast. Methylene blue acts as an electron acceptor and is reduced to its colourless leuco form. The leuco dye is oxidized by oxygen present in the solution producing a reversible system. Reproducible signals are obtained if the solution is saturated with oxygen. The measuring range depends on the concentration of methylene blue and has to be adapted to the measuring problem. Lactate determinations in real samples by the new method agreed with determinations made by the classical NADH method. Springer-Verlag, 1990 Rohen Anja Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut Krause Jörg Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut Cammann Karl Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/2(1990-01-01), 168-171 0937-0633 338:2<168 1990 338 216 https://doi.org/10.1007/BF00321882 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00321882 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rohen Anja Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Krause Jörg Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Cammann Karl Lehrstuhl für Analytische Chemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Strasse 8, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 773 0- Fresenius' Journal of Analytical Chemistry Springer-Verlag 338/2(1990-01-01), 168-171 0937-0633 338:2<168 1990 338 216 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer