caa a22 4500 465753027 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01600301 doi (NATIONALLICENCE)springer-10.1007/BF01600301 Histological and ultrastructural effects of cyclosporin A on normal human skin xenografted on to nude mice [Elektronische Daten] [Jean Kanitakis, Ana Ramirez-Bosca, Marek Haftek, Jean Thivolet] Summary: Cyclosporin A (CsA) is a potent immunosuppressant with a selective activity on T-helper lymphocytes. However, CsA also exerts biological effects on non-lymphoid cells (fibroblasts, endothelial and epithelial cells). CsA can inhibit in vivo and in vitro DNA synthesis of epidermal keratinocytes (EK) and induces in vivo morphological alterations of kidney epithelial cells. In the present study we investigated the in vivo effects of a short-term CsA treatment (50 mg/kg per day) on DNA synthesis (evaluated through 5-bromo-2′-deoxyuridine incorporation) and on the histological features of normal human skin xenografted (NHSX) on to congenitally athymic nude mice. When compared with control NHSX, CsA induced a statistically significant inhibition of DNA synthesis of NHSX EK. At the light- and electron-microscopic level, apart from a decrease in the thickness of the viable epidermis of NHSX (statistically non-significant), no noticeable differences between treated and control NHSX could be detected. EK, Langerhans cells and melanocytes appeared morphologically unaffected by CsA and no signs of acute toxicity (giant mitochondria, vacuolization, microcalcifications) were seen. These results suggest that CsA exerts a subtle effect on human EK; indeed, despite an unequivocal antiproliferative activity, no significant histological changes related to the acute CsA toxicity seem to be induced on the various epidermal cell types. Springer-Verlag, 1990 Cyclosporin A nationallicence Human skin xenografts nationallicence Histology nationallicence Electron microscopy nationallicence Kanitakis Jean INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut Ramirez-Bosca Ana INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut Haftek Marek INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut Thivolet Jean INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 505-511 0174-7398 416:6<505 1990 416 428 https://doi.org/10.1007/BF01600301 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600301 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanitakis Jean INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Ramirez-Bosca Ana INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Haftek Marek INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Thivolet Jean INSERM U 209/CNRS URA 189, Hôpital Ed. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 505-511 0174-7398 416:6<505 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer