caa a22 4500 465753035 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01600302 doi (NATIONALLICENCE)springer-10.1007/BF01600302 Complementary expression of melanosomal antigens and constant expression of pigment-independent antigen during the evolution of melanocytic tumours [Elektronische Daten] [Hiroyuki Takahashi, Peter Parsons, Diane Favier, Max McEwan, Geoffrey Strutton, Yutaka Akutsu, Kowichi Jimbow] Summary: We have generated monoclonal antibodies (MoAbs) against melanosomal proteins (MoAb 1C11 and MoAb HMSA-1) and a cytoplasmic protein strongly synthesized in neoplastic melanocytes but not associated with melanogenesis (MoAb 7H11). An immunohistochemical study of paraffin sections showed that nearly 90% of epidermal neoplastic melanocytes, including melanomas, expressed 1C11 antigen, whereas this antigen was poorly preserved in dermal melanocytic cells except melanomas. HMSA-1 antigen was expressed in a complementary manner to 1C11 antigen, being found in dermal naevus cells but not generally in the epidermal regions, except for dysplastic naevi and melanomas. In contrast, 7H11 antigen was distributed in nearly 90% of melanocytic tumours except solar lentigo and lentigo maligna lesions. The failure of MoAb 1C11 to react with dermal melanocytes may reflect a subtle alteration in melanogenesis during tumour evolution. Overall, the combined use of MoAbs serves as an accurate diagnosis of melanocytic tumours, the pigment-independent MoAb 7H11 being particularly useful for amelanotic and metastatic lesions. Springer-Verlag, 1990 Monoclonal antibody nationallicence Tyrosinase nationallicence Malignant melanoma nationallicence Metastasis nationallicence Immunohistochemistry nationallicence Takahashi Hiroyuki Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut Parsons Peter Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut Favier Diane Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut McEwan Max Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut Strutton Geoffrey Department of Pathology, Royal Brisbane Hospital, Herston, Queensland, Australia aut Akutsu Yutaka Department of Dermatology, Sapporo Medical College, Sapporo, Japan aut Jimbow Kowichi Division of Dermatology and Cutaneous Sciences, University of Alberta, Edmonton, Canada aut Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 513-519 0174-7398 416:6<513 1990 416 428 https://doi.org/10.1007/BF01600302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Hiroyuki Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut NATIONALLICENCE 700 1- Parsons Peter Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut NATIONALLICENCE 700 1- Favier Diane Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut NATIONALLICENCE 700 1- McEwan Max Queensland Institute of Medical Research, Bramston Terrace, 4006, Herston, Queensland, Australia aut NATIONALLICENCE 700 1- Strutton Geoffrey Department of Pathology, Royal Brisbane Hospital, Herston, Queensland, Australia aut NATIONALLICENCE 700 1- Akutsu Yutaka Department of Dermatology, Sapporo Medical College, Sapporo, Japan aut NATIONALLICENCE 700 1- Jimbow Kowichi Division of Dermatology and Cutaneous Sciences, University of Alberta, Edmonton, Canada aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 513-519 0174-7398 416:6<513 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer