caa a22 4500 465753043 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01600300 doi (NATIONALLICENCE)springer-10.1007/BF01600300 Identification of cytomegalovirus infection in acquired immunodeficiency syndrome [Elektronische Daten] [Tatsuo Tomita, Masahiro Chiga, Margaret Lenahan, N. Balachandran] Summary: Cytomegalovirus (CMV) infection was observed in 10 of 12 autopsy cases of acquired immunodeficiency syndrome (AIDS) and appears to be the commonest life-threatening viral infection in AIDS. In all 10 cases, adrenal glands were affected with CMV and adrenal medullary necrosis was present in 6 cases. Lungs were affected with CMV in 7 cases with disseminated infection and positive CMV culture. In situ hybridization of tissue sections with CMV-specific DNA provided positive staining for CMV in inclusions as well as other infected cells without obvious inclusions. Human diploid lung fibroblasts were infected with isolated CMV in culture, yielding positive CMV identification within 5 days by in situ hybridization before specific cytopathic changes appeared in the fibroblasts. The early and specific detection of CMV is possible by in situ hybridization with cultured fibroblasts. Springer-Verlag, 1990 Cytomegalovirus nationallicence AIDS nationallicence Adrenals nationallicence Immunohistochemical staining nationallicence In situ hybridization nationallicence Tomita Tatsuo Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut Chiga Masahiro Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut Lenahan Margaret Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut Balachandran N. Department of Microbiology, University of Kansas Medical Center, Kansas City, Kansas, USA aut Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 497-503 0174-7398 416:6<497 1990 416 428 https://doi.org/10.1007/BF01600300 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600300 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomita Tatsuo Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut NATIONALLICENCE 700 1- Chiga Masahiro Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut NATIONALLICENCE 700 1- Lenahan Margaret Department of Pathology, University of Kansas Medical Center, 66103, Kansas City, Kansas, USA aut NATIONALLICENCE 700 1- Balachandran N. Department of Microbiology, University of Kansas Medical Center, Kansas City, Kansas, USA aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 497-503 0174-7398 416:6<497 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer