caa a22 4500 465753078 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01600298 doi (NATIONALLICENCE)springer-10.1007/BF01600298 Cytokeratin-immunoreactive cells of human lymph nodes and spleen in normal and pathological conditions [Elektronische Daten] An immunocytochemical study [Claudio Doglioni, Patrizia Dell'Orto, Gianfranco Zanetti, Paolo Iuzzolino, Guido Coggi, Giuseppe Viale] Summary: The occurrence and the distribution of cytokeratin (CK)-immunoreactive reticulum cells in a series of normal and pathological human lymph nodes and spleens are documented. The immunoreactive cells exhibit morphological and immunophenotypic features of so-called fibroblastic reticulum cells, with or without myoid differentiation. They invariably co-express vimentin and, to a lesser extent, desmin and muscle-specific actin isoforms. These CK-immunoreactive cells are apparently a normal subpopulation of reticulum cells, being detectable from the early stages of spleen and lymph node development. They are distributed mainly in the paracortical and medullary regions of the lymph nodes and at the periphery of the white pulp in the spleen. Their number and distribution are highly variable in different neoplastic and non-neoplastic pathological conditions but the changes are not disease specific. CK-immunoreactive reticulum cells are easily identifiable in both frozen and fixed lymphoid tissue and in cytological smears of fine-needle aspirates, provided that monoclonal antibodies whose spectrum of reactivity includes cytokeratins 8 and 18 are used. The awareness of the occurrence of CK-immunoreactive cells in normal lymphoid tissues is of particular relevance in the search for micrometastatic foci using anti-CK antibodies. Springer-Verlag, 1990 Cytokeratins nationallicence Reticulum cells nationallicence Lymph nodes nationallicence Spleen nationallicence Doglioni Claudio Department of Surgical Pathology, Ospedale Civile, 32032, Feltre, Italy aut Dell'Orto Patrizia Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut Zanetti Gianfranco Department of Surgical Pathology, Ospedale Civile, 32032, Feltre, Italy aut Iuzzolino Paolo Ospedale Borgo Trento, Verona aut Coggi Guido Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut Viale Giuseppe Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 479-490 0174-7398 416:6<479 1990 416 428 https://doi.org/10.1007/BF01600298 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600298 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doglioni Claudio Department of Surgical Pathology, Ospedale Civile, 32032, Feltre, Italy aut NATIONALLICENCE 700 1- Dell'Orto Patrizia Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut NATIONALLICENCE 700 1- Zanetti Gianfranco Department of Surgical Pathology, Ospedale Civile, 32032, Feltre, Italy aut NATIONALLICENCE 700 1- Iuzzolino Paolo Ospedale Borgo Trento, Verona aut NATIONALLICENCE 700 1- Coggi Guido Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut NATIONALLICENCE 700 1- Viale Giuseppe Second Department of Pathology, University of Milan School of Medicine, Milan, Italy aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/6(1990-11-01), 479-490 0174-7398 416:6<479 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer