caa a22 4500 465753191 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01678978 doi (NATIONALLICENCE)springer-10.1007/BF01678978 Dendritic reticulum cell-related immunostaining for laminin in follicular and diffuse B-cell lymphomas [Elektronische Daten] [Annunziata Gloghini, Antonino Carbone] Summary: We performed a comparative immunohisto-cytochemical study of the distribution patterns of laminin and follicular dendritic reticulum cells (DRCs) within their follicular microenvironment in both nodular or diffuse B-cell non Hodgkin's lymphomas (NHLs). Twenty nine cases of immunophenotypically diagnosed B-cell NHLs (19 of follicular center cell origin-FCCL- and 10 of the diffuse well differentiated lymphocytic type-WDLL-) and five reactive lymph nodes with follicular hyperplasia were analyzed by immunoperoxidase and immunofluorescence techniques. Serial frozen sections and cytospin preparations were tested either with single antibodies anti laminin and DRC-1, or paired reagents in double labeling immunofluorescence. Our results indicated consistently that within both the reactive germinal centers and the neoplastic nodules of FCCL laminin immunostaining visualized a punctate-granular pattern apart from the linear vascular basement membrane positivity. Double immunofluorescence assay demonstrated that there was a close parallelism between this laminin staining pattern and DRC-1 distribution showing a well developed DRCs meshwork; in the diffuse tumour areas of both FCCL and WDLL, laminin immunoreactivity was found only in those cases in which nests of DRCs were observed. Double immunofluorescence studies performed on cytospin preparations demonstrated that the groups of cells containing DRC-1 positive cells, contained a positivity for laminin, although within the cell the staining for DRC-1 was intense and diffuse, while that for laminin was granular and more sparse. Our results suggested that these laminin and DRC-1 positive reactive sites may be present on the same cells. Since the reduction in number or loss of both DRCs and their related immunostaining for laminin within the microenvironment was consistently associated with a loss of nodularity by lymphoma cells, whereas nodularity in reactive and neoplastic conditions was associated with a rich DRCs meshwork and the related laminin immunostaining, a trapping function of DRCs exercised in the presence of laminin should be considered. Springer-Verlag, 1990 Laminin nationallicence Basement membrane nationallicence Follicular dendritic reticulum cell nationallicence B-cell lymphoma nationallicence Immunohistology nationallicence Immunofluorescence nationallicence Gloghini Annunziata Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut Carbone Antonino Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut Virchows Archiv A Springer-Verlag 416/3(1990-05-01), 197-204 0174-7398 416:3<197 1990 416 428 https://doi.org/10.1007/BF01678978 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01678978 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gloghini Annunziata Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut NATIONALLICENCE 700 1- Carbone Antonino Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/3(1990-05-01), 197-204 0174-7398 416:3<197 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer