caa a22 4500 46575323X CHVBK 20180323111839.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01605291 doi (NATIONALLICENCE)springer-10.1007/BF01605291 The surface to volume ratio of mitochondria, a suitable parameter for evaluating mitochondrial swelling [Elektronische Daten] Correlations during the course of myocardial global ischaemia [A. Schmiedl, Ph. Schnabel, G. Mall, M. Gebhard, D. Hunneman, J. Richter, H. Bretschneider] Summary: Cellular changes occuring in the left ventricular myocardium during ischaemia after different methods of cardiac arrest have been evaluated by morphological and morphometric parameters: volume densities of mitochondria (VVMi), sarcoplasm (VVSp), myofibrils (VVMf), surface densities of mitochondria (SVMi). The surface to volume ratio of mitochondria (SVratioMi) has been used as an independent parameter of mitochondrial swelling. Since ischaemic swelling of myocardial cells increases the volume of the reference space and ischaemic swelling of mitochondria decreases the free sarcoplasm, VVMi and VVSp cannot be considered as reliable indicators of the degree of oedema. SVMi/VVMf remains nearly constant after different forms of cardiac arrest, demonstrating the integrity of mitochondrial outer membranes. The inverse linear ratio between SVratioMi and the mean mitochondrial volume indicates that the increase in mitochondrial volume is achieved by surface smoothing. Loss of matrix structure and fragmentation of cristae occur at an SVratioMi of about 5.8, cristolysis at 5.5 to 5.6 and amorphous matrix densities at an SVratioMi of less than 5.5 μm2/μm3. The SVratioMi is a suitable parameter for evaluating mitochondrial swelling both at the onset and during global myocardial ischaemia, independent of the method of cardiac arrest used. It serves as an indicator of the state of structural preservation of mitochondria during ischaemia. Springer-Verlag, 1990 Myocardial ultrastructure nationallicence Mitochondrial swelling nationallicence Stereology nationallicence Correlations of structural parameters nationallicence Cardiac arrest and global ischaemia nationallicence Schmiedl A. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut Schnabel Ph Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut Mall G. Pathologisches Institut der Universität Heidelberg, Federal Republic of Germany aut Gebhard M. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut Hunneman D. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut Richter J. Zentrum Anatomie der Universität Göttingen, Federal Republic of Germany aut Bretschneider H. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut Virchows Archiv A Springer-Verlag 416/4(1990-07-01), 305-315 0174-7398 416:4<305 1990 416 428 https://doi.org/10.1007/BF01605291 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605291 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmiedl A. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schnabel Ph Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Mall G. Pathologisches Institut der Universität Heidelberg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Gebhard M. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hunneman D. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Richter J. Zentrum Anatomie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bretschneider H. Zentrum Physiologie und Pathophysiologie der Universität Göttingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/4(1990-07-01), 305-315 0174-7398 416:4<305 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer