caa a22 4500 465753248 CHVBK 20180323111839.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01605293 doi (NATIONALLICENCE)springer-10.1007/BF01605293 Phenotypic characteristics of tumour-infiltrating lymphocytes in human oesophageal cancer tissues defined by quantitative two-colour analysis with flow-cytometry [Elektronische Daten] [Hideaki Tahara, Hitoshi Shiozaki, Kenji Kobayashi, Tokiharu Yano, Hiroshi Yano, Shigeyuki Tamura, Kunihiko Oku, Mikiyo Miyata, Ken'ichi Wakasa, Masami Sakurai, Takesada Mori] Summary: Phenotypic subpopulations of tumourinfiltrating lymphocytes (TIL) separated from human oesophageal cancer tissues were defined by quantitative two-colour analysis with flow-cytometry (FACScan), and their characteristics were investigated by comparison with peripheral blood lymphocytes (PBL) and intra-oesophageal lymphocytes from noncancerous tissue (IEL) as the controls. Fifteen patients (13 males and 2 females) with squamous cell carcinoma of the oesophagus were entered into the study. Lymphocytes were analyzed by FACScan and the frequencies of the subpopulations were determined using monoclonal antibodies for surface markers. Single colour analysis revealed a predominance of T cells among TIL and a significant reduction of natural killer (NK) cells compared with the controls. Two-colour analysis showed that CD4+Leu8− (helper T cells) and CD8+CD11b− (cytotoxic T cells) were significantly increased among TIL when compared with the controls. This significant increase of both helper and cytotoxic T cells, which are indispensable components of cellular immunity, strongly implies that TIL are performing a role in the expression of antigen-specific cellular immunity against the tumours. This is the first report of a phenotypic study of human oesophageal cancer that clearly indicates the significance of the TIL and suggests their potential for use as a source of adoptive immunotherapy. Springer-Verlag, 1990 Oesophageal cancer nationallicence Tumour infiltrating lymphocyte nationallicence Phenotypic subpopulation nationallicence Cell-mediated immunity nationallicence Flow-cytometry nationallicence Tahara Hideaki Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Shiozaki Hitoshi Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Kobayashi Kenji Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Yano Tokiharu Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Yano Hiroshi Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Tamura Shigeyuki Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Oku Kunihiko Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Miyata Mikiyo Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Wakasa Ken'ichi Department of Pathology, Osaka University Hospital, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Sakurai Masami Department of Pathology, Osaka University Hospital, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Mori Takesada Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut Virchows Archiv A Springer-Verlag 416/4(1990-07-01), 329-334 0174-7398 416:4<329 1990 416 428 https://doi.org/10.1007/BF01605293 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605293 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tahara Hideaki Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Shiozaki Hitoshi Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Kobayashi Kenji Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Yano Tokiharu Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Yano Hiroshi Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Tamura Shigeyuki Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Oku Kunihiko Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Miyata Mikiyo Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Wakasa Ken'ichi Department of Pathology, Osaka University Hospital, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Sakurai Masami Department of Pathology, Osaka University Hospital, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 700 1- Mori Takesada Second Department of Surgery, Osaka University Medical School, 1-1-50, Fukushima, Fukushima-ku, 553, Osaka City, Japan aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/4(1990-07-01), 329-334 0174-7398 416:4<329 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer