caa a22 4500 465753353 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01605145 doi (NATIONALLICENCE)springer-10.1007/BF01605145 Immunohistochemical study of the abnormal cells in Langerhans cell histiocytosis (Histiocytosis x) [Elektronische Daten] [K. Ornvold, E. Ralfkiaer, H. Carstensen] Summary: The immunophenotypic properties of the abnormal cells in routine specimens from 16 cases of Langerhans cell histiocytosis (LCH) were examined. In five cases, cryostat sections were also available. The abnormal cells expressed a similar phenotype and were positive for HLA-DR, S-100 protein, peanut agglutinin (PNA), CD1a, CD4 and several macrophage-associated markers, including CD11c, CDw32 and CD68 (the latter detectable in routine sections with antibody KP1). Staining with CD14, CD35 (C3b receptor), and CD11b (C3bi receptor) was negative with the exception of one of the cases in which a proportion of the cells showed faint positivity with CD11b. Staining for pan-T-cell (CD2, CD3, CD5) and panB-cell (CD 19, CD22) antigens was negative in all lesions. It is concluded that LCH expresses a characteristic phenotype with some heterogeneity with regard to macrophage markers and that immunohistochemical methods in cryostat sections and routine specimens form a useful supplement to other techniques for the diagnosis of this condition. Springer-Verlag, 1990 Langerhans cell histiocytosis nationallicence Immunohistochemistry nationallicence Ornvold K. Laboratory of Paediatric Pathology, Rigshospitale, University Hospital, Copenhagen, Denmark aut Ralfkiaer E. Department of Pathology, Rigshospitale, University Hospital, Copenhagen, Denmark aut Carstensen H. Department of Paediatrics, Rigshospitale, University Hospital, Copenhagen, Denmark aut Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 403-410 0174-7398 416:5<403 1990 416 428 https://doi.org/10.1007/BF01605145 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605145 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ornvold K. Laboratory of Paediatric Pathology, Rigshospitale, University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Ralfkiaer E. Department of Pathology, Rigshospitale, University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Carstensen H. Department of Paediatrics, Rigshospitale, University Hospital, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 403-410 0174-7398 416:5<403 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer