caa a22 4500 465753361 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01605149 doi (NATIONALLICENCE)springer-10.1007/BF01605149 Haemopoiesis in human fetal and embryonic liver [Elektronische Daten] Immunohistochemical determination in B5-fixed paraffin-embedded tissues [Wim Timens, Willem Kamps, Thea Rozeboom-Uiterwijk, Sibrand Poppema] Summary: Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes and their microenvironment. Seven embryonal and twelve fetal liver specimens were studied, having been fixed in B5-fixative and embedded in paraffin. The different haemopoietic lineages each showed their own immunophenotype and distribution; intercellular and microenvironmental relationships were easily determined. Erythroid cells are reactive with VIE-G4, LN1, and MT1, sometimes partly surrounding a central macrophage. Myelomonocytic cells react with LCA, MT1, MB3, LN2, and anti-lysozyme, and from 14 weeks onwards with LN3. Lymphoid cells show LCA, MT1, MT2, MB1, MB2, MB3, and LN2 reactivity. In a few cases some scarce My10+ early progenitor cells were seen. An important finding is the extensive MT1-reactivity distributed over all haemopoietic lineages, and the demonstration of immature haemopoietic blast cells exclusively expressing the MT1 antigen. Further studies employing MT1 are necessary to delineate the extent of the distribution and the possible function of the antigen. Use of the MT1 mAb may contribute to the elucidation of the exact nature of the haemopoietic blast cells and their place in haemopoietic development. Springer-Verlag, 1990 Immunohistology nationallicence Monoclonal antibodies nationallicence MT1 nationallicence Progenitor cells nationallicence Haematopoiesis nationallicence Timens Wim Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut Kamps Willem Department of Pediatric Oncology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut Rozeboom-Uiterwijk Thea Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut Poppema Sibrand Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 429-436 0174-7398 416:5<429 1990 416 428 https://doi.org/10.1007/BF01605149 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605149 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Timens Wim Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut NATIONALLICENCE 700 1- Kamps Willem Department of Pediatric Oncology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut NATIONALLICENCE 700 1- Rozeboom-Uiterwijk Thea Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut NATIONALLICENCE 700 1- Poppema Sibrand Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 429-436 0174-7398 416:5<429 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer