caa a22 4500 465753418 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01605146 doi (NATIONALLICENCE)springer-10.1007/BF01605146 Analysis of the tumour-associated antigen TAG-12 by monoclonal antibody 7A9 in normal, benign and malignant mammary tissues [Elektronische Daten] [M. Werner, R. von Wasielewski, J. Bernhards, A. Georgii] Summary: A monoclonal antibody 7A9 was raised against the tumour-associated glycoprotein TAG-12 purified from T47-D breast carcinoma cells. In immunoblots from cytosol of T47-D cells and from sera of breast cancer patients, antibody 7A9 detects the high molecular weight mucin-like TAG-12 antigen. A series of paraffin sections of normal, benign and malignant mammary tissues have been studied with monoclonal antibody 7A9 and the immunoalkaline phosphatase method. In resting gland, proliferating gland and fibroadenoma ducts, reactivity of 7A9 was mainly restricted to luminal membranes of epithelial cells and secretions. 77/79 primary breast carcinomas including ductal, lobular and various other carcinoma types showed cytoplasmic and/or membrane-associated staining with 7A9 in most tumour cells. Metastases (31/31) from different sites were also positive. Strong immunoreactivity with single tumour cells was noted in cytological preparations from freshly resected breast cancer tissue. Thus, monoclonal antibody 7A9 seems to be very useful for the targeting of breast carcinoma cells. Springer-Verlag, 1990 Tumour-associated antigen nationallicence Monoclonal antibody nationallicence Breast carcinoma nationallicence Werner M. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut von Wasielewski R. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut Bernhards J. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut Georgii A. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 411-416 0174-7398 416:5<411 1990 416 428 https://doi.org/10.1007/BF01605146 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605146 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Werner M. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut NATIONALLICENCE 700 1- von Wasielewski R. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut NATIONALLICENCE 700 1- Bernhards J. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut NATIONALLICENCE 700 1- Georgii A. Pathologisches Institut der Medizinischen Hochschule Hannover, Konstanty-Gutschowstrasse 8, D-3000, Hannover 61, Germany aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 411-416 0174-7398 416:5<411 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer