caa a22 4500 465753477 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01605142 doi (NATIONALLICENCE)springer-10.1007/BF01605142 Scanning electron microscopic evidence for neural differentiation in Ewing's sarcoma cell lines [Elektronische Daten] [Samuel Navarro, Manuel González-Devesa, Antonio Ferrández-Izquierdo, Timothy Triche, Antonio Llombart-Bosch] Summary: A number of recent studies have suggested a relationship between Ewing's sarcoma (ES) and other small round cell tumours of childhood such as peripheral neuroepithelioma (PN). We report scanning electron microscopic studies on the character of induced neural differentiation in ES, neuroblastoma, PN, osteosarcoma and colon carcinoma. We found evidence of neural differentiation in both neural lines and in one of two Ewing's lines before treatment. After differentiation, both Ewing's and neural lines developed neuritic processes with varicosities and little arborization, except for the initially undifferentiated Ewing's line (A4573) which displayed extensive lateral sprouting from neuritic processes after differentiation. Neither treated nor untreated osteosarcoma or colon carcinoma displayed any evidence of neural differentiation. Further, neuroblastoma cells are easily distinguished from ES and PN by virtue of their single, unbranched neuntes and lack of lateral sprouting or filopodia. These results provide further evidence for the neural character and close relationship between ES and PN. Springer-Verlag, 1990 Ewing's sarcoma nationallicence Peripheral neuroepithelioma nationallicence Scanning electron microscopy nationallicence Cell lines nationallicence Cyclic AMP nationallicence Navarro Samuel Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut González-Devesa Manuel Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut Ferrández-Izquierdo Antonio Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut Triche Timothy National Cancer Institute, Bethesda, Maryland, USA aut Llombart-Bosch Antonio Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 383-391 0174-7398 416:5<383 1990 416 428 https://doi.org/10.1007/BF01605142 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01605142 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Navarro Samuel Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- González-Devesa Manuel Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- Ferrández-Izquierdo Antonio Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut NATIONALLICENCE 700 1- Triche Timothy National Cancer Institute, Bethesda, Maryland, USA aut NATIONALLICENCE 700 1- Llombart-Bosch Antonio Department of Pathology, Medical School, University of Valencia, Avd. Blasco Ibañez n∘ 17, 46010, Valencia, Spain aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 416/5(1990-09-01), 383-391 0174-7398 416:5<383 1990 416 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer