caa a22 4500 465753515 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01600110 doi (NATIONALLICENCE)springer-10.1007/BF01600110 Relationships among expression, transcription and rearrangement of T-cell receptor Β gene in T-cell lymphomas [Elektronische Daten] [Kiyoshi Kasai, Toru Kameya, Masao Ono, Chieki Wada, Sadahito Kuwao, Tadashi Motoori] Summary: The expression of T-cell receptors (TCR) in malignant lymphomas was examined immunohistochemically by monoclonal antibodies which react with the TCRΒ or TCRδ chain. TCRΒ was expressed in 16 out of 47 non-Hodgkin's lymphomas. These included 15 T-cell lymphomas and 1 Ki-1 lymphoma. The anti-TCRΒ chain antibody,ΒF1, did not react with 26 B-cell lymphomas, 1 Ki-1 lymphoma or 6 Hodgkin's disease. The anti-TCRδ chain antibody, TCRδ1, did not react with any type of malignant lymphoma. Although TCRΒ and CD3 were co-expressed in normal lymphoid tissues and most T-cell lymphomas, 3 cases of CD3+CD4+ CD8−T-cell lymphoma failed to express TCR0. TCRΒ and Ig JH gene configurations in malignant lymphomas were examined by Southern hybridization. Although each of 9 T-cell lymphomas had a rearranged TCRΒ locus, TCRΒ gene rearrangement in the 3 cases ofΒF1−CD3+T-cell lymphomas was demonstrated by Southern blot. No transcripts of the TCRΒ gene could be found in 2 out of the 3ΒF1−CD3+T-cell lymphomas by Northern blot, indicating the lack of TCRΒ protein expression to be due to non-transcription of the TCR gene. Loss of TCRΒ proteins in these T-cell lymphomas is thus quite likely to be associated with T-cell tumour activation and progression, since 3ΒF1−CD3+T-cell lymphomas expressed CD25 (interleukin-2 receptor) to a high degree. Springer-Verlag, 1990 T-cell receptors nationallicence Malignant lymphomas nationallicence Immunohistochemistry nationallicence Transcription nationallicence Rearrangement nationallicence Kasai Kiyoshi Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Kameya Toru Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Ono Masao Departments of Molecular Biology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Wada Chieki Departments ofClinical Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Kuwao Sadahito Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Motoori Tadashi Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 57-65 0174-7398 417:1<57 1990 417 428 https://doi.org/10.1007/BF01600110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kasai Kiyoshi Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 700 1- Kameya Toru Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 700 1- Ono Masao Departments of Molecular Biology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 700 1- Wada Chieki Departments ofClinical Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 700 1- Kuwao Sadahito Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 700 1- Motoori Tadashi Departments of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Japan aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 57-65 0174-7398 417:1<57 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer