caa a22 4500 46575354X CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01600104 doi (NATIONALLICENCE)springer-10.1007/BF01600104 Three-dimensional identification of actin filaments in phalloidin-treated rat livers by quick-freezing and deep-etching method [Elektronische Daten] [Atsuhiko Naramoto, Shinichi Ohno, Nobuo Itoh, Hiroya Takami, Kou Nakazawa, Hidekazu Shigematsu] Summary: An increase in microfilaments in phalloidintreated hepatocytes of Wistar rats was identified threedimensionally with myosin subfragment 1 (S1) on replica membranes, using the quick-freezing and deep-etching method. Almost all of the reticular microfilaments around the bile canaliculi and beneath the lateral cell membranes were decorated on their surfaces by S1 attachment. Some showed periodic structures. However, thinner filaments with diameters of 4-7 nm were not decorated by S1. Bundled intermediate filaments around the bile canalicular microfilaments and intermediate filaments localized among cell organelles had smooth surfaces without S1 decoration. The microfilaments decorated by S1 were attached directly to bundled intermediate filaments. The quick-freezing and deep-etching method is useful in analysing cytoskeletal pathology and can be applied to histochemical fields. Springer-Verlag, 1990 Quick-freezing nationallicence Deep-etching nationallicence Myosin subfragment 1 nationallicence Microfilament nationallicence Intermediate filament nationallicence Naramoto Atsuhiko Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Ohno Shinichi Department of Anatomy, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Itoh Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Takami Hiroya Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Nakazawa Kou Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Shigematsu Hidekazu Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 15-20 0174-7398 417:1<15 1990 417 428 https://doi.org/10.1007/BF01600104 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600104 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Naramoto Atsuhiko Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Ohno Shinichi Department of Anatomy, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Itoh Nobuo Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Takami Hiroya Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Nakazawa Kou Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Shigematsu Hidekazu Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 15-20 0174-7398 417:1<15 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer