caa a22 4500 465753558 CHVBK 20180323111840.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01600108 doi (NATIONALLICENCE)springer-10.1007/BF01600108 Anti-vimentin antibody reactivity with Reed-Sternberg cells of Hodgkin's disease [Elektronische Daten] [Antonino Carbone, Annunziata Gloghini, Rachele Volpe, Mauro Boiocchi] Summary: There are few data on the reactivity of Reed-Sternberg (RS) cells with antibodies against vimentin. In a preliminary survey of biopsy specimens from 16 cases of Hodgkin's disease (HD), we found that the antivimentin (V9) monoclonal antibody stained RS cells in 6 cases. We therefore examined vimentin expression on RS cells immunohistologically in 38 Bouin-fixed and paraffin-embedded lymph nodes with HD [lymphocyte predominance (LP) 4; nodular sclerosis (NS) 23; mixed cellularity (MC) 7; lymphocyte depletion (LD) 4]. The results were correlated with the histopathological features, the immunohistological phenotype of the RS cells, and the findings obtained from molecular genetics studies (available in 13 cases). RS cells were found to express strong and diffuse cytoplasmic staining for vimentin in 13 cases, all of the NS subtype. No differences in antigenic expression on RS cells were found between the vimentin-positive and negative cases within the NS subtype. DNA analysis revealed no B- or T-cell clonal populations in the tested samples. The results indicated that RS cells were immunostained by anti-vimentin (V9) antibody with a relatively high frequency, but only in the NS subtype of HD. This subtype, however, was heterogeneous according to vimentin immunostaining on RS cells. The significance of this finding concerning the RS cell origin in this subset is discussed. Springer-Verlag, 1990 Reed-Sternberg cells nationallicence Vimentin nationallicence Lymph node pathology nationallicence Immunohistology nationallicence DNA analysis nationallicence Carbone Antonino Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut Gloghini Annunziata Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut Volpe Rachele Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut Boiocchi Mauro Divisions of Experimental Oncology, Centro di Riferimento Oncologico, Aviano, Italy aut Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 43-48 0174-7398 417:1<43 1990 417 428 https://doi.org/10.1007/BF01600108 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01600108 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carbone Antonino Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut NATIONALLICENCE 700 1- Gloghini Annunziata Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut NATIONALLICENCE 700 1- Volpe Rachele Division of Pathology, Centro di Riferimento Oncologico, Via Pedemontana Occidentale, I-33081, Aviano, Italy aut NATIONALLICENCE 700 1- Boiocchi Mauro Divisions of Experimental Oncology, Centro di Riferimento Oncologico, Aviano, Italy aut NATIONALLICENCE 773 0- Virchows Archiv A Springer-Verlag 417/1(1990-01-01), 43-48 0174-7398 417:1<43 1990 417 428 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer